RRC ID 27307
著者 Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O.
タイトル Structural basis for the counter-transport mechanism of a H+/Ca2+ exchanger.
ジャーナル Science
Abstract Ca(2+)/cation antiporters catalyze the exchange of Ca(2+) with various cations across biological membranes to regulate cytosolic calcium levels. The recently reported structure of a prokaryotic Na(+)/Ca(2+) exchanger (NCX_Mj) revealed its overall architecture in an outward-facing state. Here, we report the crystal structure of a H(+)/Ca(2+) exchanger from Archaeoglobus fulgidus (CAX_Af) in the two representatives of the inward-facing conformation at 2.3 Å resolution. The structures suggested Ca(2+) or H(+) binds to the cation-binding site mutually exclusively. Structural comparison of CAX_Af with NCX_Mj revealed that the first and sixth transmembrane helices alternately create hydrophilic cavities on the intra- and extracellular sides. The structures and functional analyses provide insight into the mechanism of how the inward- to outward-facing state transition is triggered by the Ca(2+) and H(+) binding.
巻・号 341(6142)
ページ 168-72
公開日 2013-7-12
DOI 10.1126/science.1239002
PII science.1239002
PMID 23704374
MeSH Antiporters / chemistry* Antiporters / genetics Antiporters / metabolism Archaeal Proteins / chemistry* Archaeal Proteins / genetics Archaeal Proteins / metabolism Archaeoglobus fulgidus / metabolism* Binding Sites Calcium / chemistry Calcium / metabolism Cation Transport Proteins / chemistry* Cation Transport Proteins / genetics Cation Transport Proteins / metabolism Crystallography, X-Ray Hydrogen / chemistry Hydrogen / metabolism Protein Structure, Secondary Protein Structure, Tertiary
IF 41.846
引用数 47
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 Genomic DNA of Archaeoglobus fulgidus JCM 9628T (JGD06275)
一般微生物 JCM 9628