RRC ID 27312
Author de León N, Sharifmoghadam MR, Hoya M, Curto MÁ, Doncel C, Valdivieso MH.
Title Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe.
Journal PLoS ONE
Abstract The regulation of cell wall synthesis by the clathrin light chain has been addressed. Schizosaccharomyces pombe clc1Δ mutant was inviable in the absence of osmotic stabilization; when grown in sorbitol-supplemented medium clc1Δ cells grew slowly, formed aggregates, and had strong defects in morphology. Additionally, clc1Δ cells exhibited an altered cell wall composition. A mutant that allowed modulating the amount of Clc1p was created to analyze in more detail the dependence of cell wall synthesis on clathrin. A 40% reduction in the amount of Clc1p did not affect acid phosphatase secretion and bulk lipid internalization. Under these conditions, β(1,3)glucan synthase activity and cell wall synthesis were reduced. Also, the delivery of glucan synthases to the cell surface, and the secretion of the Eng1p glucanase were defective. These results suggest that the defects in the cell wall observed in the conditional mutant were due to a defective secretion of enzymes involved in the synthesis/remodelling of this structure, rather than to their endocytosis. Our results show that a reduction in the amount of clathrin that has minor effects on general vesicle trafficking has a strong impact on cell wall synthesis, and suggest that this is the reason for the lethality of clc1Δ cells in the absence of osmotic stabilization.
Volume 8(8)
Pages e71510
Published 2013
DOI 10.1371/journal.pone.0071510
PII PONE-D-13-16161
PMID 23977061
PMC PMC3747244
MeSH Cell Wall / drug effects Cell Wall / metabolism* Cell Wall / ultrastructure Clathrin Light Chains / metabolism* Endocytosis / drug effects Glucans / biosynthesis Microbial Viability* / drug effects Mutation / genetics Osmosis / drug effects Schizosaccharomyces / cytology* Schizosaccharomyces / drug effects Schizosaccharomyces / metabolism* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / metabolism* Sorbitol / pharmacology
IF 2.766
Times Cited 6
Yeast HVP2032