RRC ID 27329
Author Kurokawa N, Hirai T, Takayama M, Hiwasa-Tanase K, Ezura H.
Title An E8 promoter-HSP terminator cassette promotes the high-level accumulation of recombinant protein predominantly in transgenic tomato fruits: a case study of miraculin.
Journal Plant Cell Rep.
Abstract KEY MESSAGE:The E8 promoter-HSP terminator expression cassette is a powerful tool for increasing the accumulation of recombinant protein in a ripening tomato fruit. Strong, tissue-specific transgene expression is a desirable feature in transgenic plants to allow the production of variable recombinant proteins. The expression vector is a key tool to control the expression level and site of transgene and recombinant protein expression in transgenic plants. The combination of the E8 promoter, a fruit-ripening specific promoter, and a heat shock protein (HSP) terminator, derived from heat shock protein 18.2 of Arabidopsis thaliana, produces the strong and fruit-specific accumulation of recombinant miraculin in transgenic tomato. Miraculin gene expression was driven by an E8 promoter and HSP terminator cassette (E8-MIR-HSP) in transgenic tomato plants, and the miraculin concentration was the highest in the ripening fruits, representing 30-630 μg miraculin of the gram fresh weight. The highest level of miraculin concentration among the transgenic tomato plant lines containing the E8-MIR-HSP cassette was approximately four times higher than those observed in a previous study using a constitutive 35S promoter and NOS terminator cassette (Hiwasa-Tanase et al. in Plant Cell Rep 30:113-124, 2011). These results demonstrate that the combination of the E8 promoter and HSP terminator cassette is a useful tool to increase markedly the accumulation of recombinant proteins in a ripening fruit-specific manner.
Volume 32(4)
Pages 529-36
Published 2013-4
DOI 10.1007/s00299-013-1384-7
PMID 23306632
MeSH Arabidopsis Arabidopsis Proteins / genetics Fruit / genetics Fruit / metabolism Gene Expression Regulation, Plant Glycoproteins / biosynthesis* Glycoproteins / genetics Heat-Shock Proteins / genetics Lycopersicon esculentum / genetics* Lycopersicon esculentum / metabolism Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Promoter Regions, Genetic* Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics Terminator Regions, Genetic*
IF 3.499
Times Cited 5
Tomato TOMJPF00001