| RRC ID |
27456
|
| 著者 |
Xu L, Zhang Y, Deng ZS, Zhao L, Wei XL, Wei GH.
|
| タイトル |
Rhizobium qilianshanense sp. nov., a novel species isolated from root nodule of Oxytropis ochrocephala Bunge in China.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
During a study of the diversity and phylogeny of rhizobia isolated from root nodules of Oxytropis ochrocephala grown in the northwest of China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. These strains have identical 16S rRNA gene sequences, which showed a mean similarity of 94.4 % with the most closely related species, Rhizobium oryzae. Analysis of recA and glnA sequences showed that these strains have less than 88.1 and 88.7 % similarity with the defined species of Rhizobium, respectively. The genetic diversity revealed by ERIC-PCR fingerprinting indicated that the isolates correspond to different strains. Strain CCNWQLS01(T) contains Q-10 as the predominant ubiquinone. The major fatty acids were identified as feature 8 (C18: 1ω7c and/or C18: 1ω6c; 67.2 %). Therefore, a novel species Rhizobium qilianshanense sp. nov. is proposed, and CCNWQLS01(T) (= ACCC 05747(T) = JCM 18337(T)) is designated as the type strain.
|
| 巻・号 |
103(3)
|
| ページ |
559-65
|
| 公開日 |
2013-3-1
|
| DOI |
10.1007/s10482-012-9840-x
|
| PMID |
23142858
|
| MeSH |
Bacterial Typing Techniques
China
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Glutamate-Ammonia Ligase / genetics
Molecular Sequence Data
Molecular Typing
Oxytropis / microbiology*
Phylogeny
Polymerase Chain Reaction
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Rec A Recombinases / genetics
Rhizobium / chemistry
Rhizobium / classification*
Rhizobium / genetics
Rhizobium / isolation & purification*
Root Nodules, Plant / microbiology*
Sequence Analysis, DNA
|
| IF |
2.674
|
| 引用数 |
13
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 18337 |
