Reference - Detail
| RRC ID | 27492 |
|---|---|
| Author | Shinozaki Y, Morita T, Cao XH, Yoshida S, Koitabashi M, Watanabe T, Suzuki K, Sameshima-Yamashita Y, Nakajima-Kambe T, Fujii T, Kitamoto HK. |
| Title | Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. |
| Journal | Appl Microbiol Biotechnol |
| Abstract |
Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA). An enzyme named PaE was isolated and the gene encoding PaE was cloned from the strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence of PaE contains 198 amino acids with a predicted molecular weight of 20,362.41. High identity was observed between this sequence and that of cutinase-like enzymes (CLEs) (61-68%); therefore, the gene encoding PaE was named PaCLE1. The specific activity of PaE against emulsified PBSA was 54.8±6.3 U/mg. In addition to emulsified BPs, PaE degraded solid films of PBS, PBSA, poly(ε-caprolactone), and poly(lactic acid). |
| Volume | 97(7) |
| Pages | 2951-9 |
| Published | 2013-4-1 |
| DOI | 10.1007/s00253-012-4188-8 |
| PMID | 22678026 |
| MeSH | Adipates / metabolism Amino Acid Sequence Biotransformation Butylene Glycols / metabolism Carboxylic Ester Hydrolases / chemistry Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism* Cloning, Molecular DNA, Fungal / chemistry DNA, Fungal / genetics Molecular Sequence Data Molecular Weight Plastics / metabolism* Polymers / metabolism Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae / genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Succinates / metabolism Ustilaginales / enzymology* Ustilaginales / genetics |
| IF | 3.53 |
| Times Cited | 48 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 10317 |