| RRC ID |
27492
|
| Author |
Shinozaki Y, Morita T, Cao XH, Yoshida S, Koitabashi M, Watanabe T, Suzuki K, Sameshima-Yamashita Y, Nakajima-Kambe T, Fujii T, Kitamoto HK.
|
| Title |
Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization.
|
| Journal |
Appl Microbiol Biotechnol
|
| Abstract |
Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA). An enzyme named PaE was isolated and the gene encoding PaE was cloned from the strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence of PaE contains 198 amino acids with a predicted molecular weight of 20,362.41. High identity was observed between this sequence and that of cutinase-like enzymes (CLEs) (61-68%); therefore, the gene encoding PaE was named PaCLE1. The specific activity of PaE against emulsified PBSA was 54.8±6.3 U/mg. In addition to emulsified BPs, PaE degraded solid films of PBS, PBSA, poly(ε-caprolactone), and poly(lactic acid).
|
| Volume |
97(7)
|
| Pages |
2951-9
|
| Published |
2013-4-1
|
| DOI |
10.1007/s00253-012-4188-8
|
| PMID |
22678026
|
| MeSH |
Adipates / metabolism
Amino Acid Sequence
Biotransformation
Butylene Glycols / metabolism
Carboxylic Ester Hydrolases / chemistry
Carboxylic Ester Hydrolases / genetics
Carboxylic Ester Hydrolases / metabolism*
Cloning, Molecular
DNA, Fungal / chemistry
DNA, Fungal / genetics
Molecular Sequence Data
Molecular Weight
Plastics / metabolism*
Polymers / metabolism
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Succinates / metabolism
Ustilaginales / enzymology*
Ustilaginales / genetics
|
| IF |
3.53
|
| Times Cited |
48
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 10317 |
