| RRC ID |
27503
|
| 著者 |
Chen YS, Yu CR, Ji SH, Liou MS, Leong KH, Pan SF, Wu HC, Lin YH, Yu B, Yanagida F.
|
| タイトル |
Enterocin T, a novel class IIa bacteriocin produced by Enterococcus sp. 812.
|
| ジャーナル |
Arch Microbiol
|
| Abstract |
Enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of Gram-positive bacteria, including Listeria monocytogenes. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was inactivated by protease K. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-ATYYGNGVYXDKKKXWVEWGQA, by Edman degradation, which contained the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The obtained partial sequence showed high homology with some enterococcal bacteriocins; however, no identical peptide or protein was found. This peptide was therefore considered to be a novel bacteriocin produced by Enterococcus sp. 812 and was termed enterocin T.
|
| 巻・号 |
195(9)
|
| ページ |
655-60
|
| 公開日 |
2013-9-1
|
| DOI |
10.1007/s00203-013-0917-3
|
| PMID |
23912616
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Bacteriocins / chemistry
Bacteriocins / isolation & purification*
Bacteriocins / metabolism*
Enterococcus / chemistry
Enterococcus / metabolism
Gram-Positive Bacteria
Sequence Alignment
|
| IF |
1.884
|
| 引用数 |
3
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1157 |
