| RRC ID |
27510
|
| 著者 |
Taiyoji M, Yamanaka T, Tsuno T, Ohtsubo S.
|
| タイトル |
Potential value of a rice protein extract, containing proteinaceous inhibitors against cysteine proteinases from Porphyromonas gingivalis, for managing periodontal diseases.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Arg-specific gingipain (Rgp) is a major pathogenic determinant of Porphyromonas gingivalis which is a major pathogen in periodontal disease. We prepared protein extracts with Rgp-inhibitory activity from polished rice (Oryza sativa) and evaluated the effects of these extracts on the growth and pathogenicity of P. gingivalis. The extracts inhibited the proteolytic degradation of human proteins by P. gingivalis proteinases, and repressed the growth and homotypic biofilm formation of P. gingivalis. The disruption of adhesion of epithelial cells by P. gingivalis was also restricted by the rice protein extracts. Our results suggested that the rice protein extracts suppressed the pathogenicity and growth of P. gingivalis by inhibiting the bacterial proteinase activities, implying that the Rgp-inhibitory proteins prepared from rice may be potentially valuable as nutraceutical agents for preventing periodontal diseases.
|
| 巻・号 |
77(1)
|
| ページ |
80-6
|
| 公開日 |
2013-1-1
|
| DOI |
10.1271/bbb.120585
|
| PII |
DN/JST.JSTAGE/bbb/120585
|
| PMID |
23291749
|
| MeSH |
Adhesins, Bacterial / metabolism*
Bacteroidaceae Infections / prevention & control
Biofilms / drug effects*
Biofilms / growth & development
Cell Adhesion / drug effects
Cell Line, Tumor
Cysteine Endopeptidases / metabolism*
Cysteine Proteinase Inhibitors / isolation & purification
Cysteine Proteinase Inhibitors / pharmacology*
Enzyme Assays
Epithelial Cells / cytology
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Fibroblasts / cytology
Fibroblasts / drug effects
Fibroblasts / metabolism
Gingipain Cysteine Endopeptidases
Humans
Oryza / chemistry*
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Plant Proteins / isolation & purification
Plant Proteins / pharmacology*
Porphyromonas gingivalis / drug effects*
Porphyromonas gingivalis / enzymology
Porphyromonas gingivalis / growth & development
Proteolysis / drug effects
|
| IF |
1.516
|
| 引用数 |
3
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 12257 |
| ヒト・動物細胞 |
Sa3(RCB0980) |
