RRC ID 27510
Author Taiyoji M, Yamanaka T, Tsuno T, Ohtsubo S.
Title Potential value of a rice protein extract, containing proteinaceous inhibitors against cysteine proteinases from Porphyromonas gingivalis, for managing periodontal diseases.
Journal Biosci. Biotechnol. Biochem.
Abstract Arg-specific gingipain (Rgp) is a major pathogenic determinant of Porphyromonas gingivalis which is a major pathogen in periodontal disease. We prepared protein extracts with Rgp-inhibitory activity from polished rice (Oryza sativa) and evaluated the effects of these extracts on the growth and pathogenicity of P. gingivalis. The extracts inhibited the proteolytic degradation of human proteins by P. gingivalis proteinases, and repressed the growth and homotypic biofilm formation of P. gingivalis. The disruption of adhesion of epithelial cells by P. gingivalis was also restricted by the rice protein extracts. Our results suggested that the rice protein extracts suppressed the pathogenicity and growth of P. gingivalis by inhibiting the bacterial proteinase activities, implying that the Rgp-inhibitory proteins prepared from rice may be potentially valuable as nutraceutical agents for preventing periodontal diseases.
Volume 77(1)
Pages 80-6
Published 2013
DOI 10.1271/bbb.120585
PII DN/JST.JSTAGE/bbb/120585
PMID 23291749
MeSH Adhesins, Bacterial / metabolism* Bacteroidaceae Infections / prevention & control Biofilms / drug effects* Biofilms / growth & development Cell Adhesion / drug effects Cell Line, Tumor Cysteine Endopeptidases / metabolism* Cysteine Proteinase Inhibitors / isolation & purification Cysteine Proteinase Inhibitors / pharmacology* Enzyme Assays Epithelial Cells / cytology Epithelial Cells / drug effects Epithelial Cells / metabolism Fibroblasts / cytology Fibroblasts / drug effects Fibroblasts / metabolism Humans Oryza / chemistry* Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plant Proteins / isolation & purification Plant Proteins / pharmacology* Porphyromonas gingivalis / drug effects* Porphyromonas gingivalis / enzymology Porphyromonas gingivalis / growth & development Proteolysis / drug effects
IF 1.255
Times Cited 3
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 12257