RRC ID 27529
著者 Yamauchi Y, Minegishi H, Echigo A, Shimane Y, Shimoshige H, Kamekura M, Itoh T, Doukyu N, Inoue A, Usami R.
タイトル Halarchaeum salinum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt.
ジャーナル Int J Syst Evol Microbiol
Abstract Three halophilic archaeal strains, MH1-34-1(T), MH1-16-1 and MH1-224-5 were isolated from commercial salt samples produced from seawater in Indonesia, the Philippines and Japan, respectively. Cells of the three strains were pleomorphic and stained Gram-negative. Strain MH1-34-1(T) was orange-red pigmented, while MH1-16-1 and MH1-224-5 were pink-pigmented. Strain MH1-34-1(T) was able to grow at 12-30 % (w/v) NaCl (with optimum at 18 % NaCl, w/v) at pH 4.5-7.2 (optimum, pH 5.2-5.5) and at 15-45 °C (optimum, 42 °C). Strains MH1-16-1 and MH1-224-5 grew in slightly different ranges. These strains required at least 1 mM Mg(2+) for growth. The 16S rRNA gene sequences of strains MH1-34-1(T), MH1-16-1 and MH1-224-5 were almost identical (99.8-99.9 % similarities), and the closest relative was Halarchaeum acidiphilum MH-1-52-1(T) with 98.4 % similarities. The DNA G+C contents of MH1-34-1(T), MH1-16-1 and MH1-224-5 were 59.3, 60.8 and 61.0 mol%, respectively. The level of DNA-DNA relatedness amongst the three strains was 90-91 %, while that between each of the three strains and Halarchaeum acidiphilum MH1-52-1(T) was 51-55 %. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolates should represent a novel species of the genus Halarchaeum, for which the name Halarchaeum salinum sp. nov. is proposed. The type strain is MH1-34-1(T) ( = JCM 16330(T) = CECT 7574(T)).
巻・号 63(Pt 3)
ページ 1138-1142
公開日 2013-3-1
DOI 10.1099/ijs.0.044693-0
PMID 22753525
MeSH Base Composition DNA, Archaeal / genetics Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification Indonesia Japan Molecular Sequence Data Nucleic Acid Hybridization Philippines Phylogeny* RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Sodium Chloride*
IF 2.415
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16330