| RRC ID |
27541
|
| Author |
Shojaei H, Daley C, Gitti Z, Hashemi A, Heidarieh P, Moore ERB, Naser AD, Russo C, van Ingen J, Tortoli E.
|
| Title |
Mycobacterium iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
The isolation and characterization of a novel, rapidly growing, scotochromogenic mycobacterial species is reported. Eight independent strains were isolated from clinical specimens from six different countries of the world, two in Iran, two in Italy and one in each of following countries: Greece, The Netherlands, Sweden and the USA. Interestingly, two of the strains were isolated from cerebrospinal fluid. The strains were characterized by rapid growth and presented orange-pigmented scotochromogenic colonies. DNA-based analysis revealed unique sequences in the four regions investigated: the 16S rRNA gene, the rRNA gene internal transcribed spacer 1 and the genes encoding the 65 kDa heat-shock protein and the beta-subunit of RNA polymerase. The phylogenetic analysis placed the strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium gilvum. The genotypic and phenotypic data both strongly supported the inclusion of the strains investigated here as members of a novel species within the genus Mycobacterium; the name Mycobacterium iranicum sp. nov. is proposed to indicate the isolation in Iran of the first recognized strains. The type strain is M05(T) ( = DSM 45541(T) = CCUG 62053(T) = JCM 17461(T)).
|
| Volume |
63(Pt 4)
|
| Pages |
1383-1389
|
| Published |
2013-4-1
|
| DOI |
10.1099/ijs.0.043562-0
|
| PMID |
22843713
|
| MeSH |
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Typing Techniques
Cerebrospinal Fluid / microbiology
DNA, Bacterial / genetics
DNA, Ribosomal Spacer / genetics
DNA-Directed RNA Polymerases / genetics
Fatty Acids / analysis
Female
Greece
Heat-Shock Proteins / genetics
Humans
Iran
Italy
Male
Microbial Sensitivity Tests
Middle Aged
Molecular Sequence Data
Mycobacterium / classification*
Mycobacterium / genetics
Mycobacterium / isolation & purification*
Mycobacterium Infections / microbiology
Mycolic Acids / analysis
Netherlands
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sputum / microbiology
Sweden
United States
Wounds and Injuries / microbiology
Young Adult
|
| IF |
2.415
|
| Times Cited |
26
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 17461 |
