| RRC ID |
27566
|
| Author |
Kim JY, Lee J, Shin NR, Yun JH, Whon TW, Kim MS, Jung MJ, Roh SW, Hyun DW, Bae JW.
|
| Title |
Orbus sasakiae sp. nov., a bacterium isolated from the gut of the butterfly Sasakia charonda, and emended description of the genus Orbus.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A novel Gram-stain-negative, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain C7(T), was isolated from the gut of the butterfly Sasakia charonda. Strain C7(T) grew optimally at 20-25 °C, at pH 7-8 and with 1 % (w/v) NaCl. The strain was negative for oxidase activity but positive for catalase activity. The 16S rRNA gene sequences of strain C7(T) and Orbus hercynius CN3(T) shared 96.8 % similarity. The major fatty acids identified were C14 : 0, C16 : 0, C18 : 1ω7c and summed feature 2 (comprising C14 : 0 3-OH/iso-C16 : 1). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain C7(T) were phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid and two unidentified aminophospholipids. The G+C content of the genomic DNA extracted from strain C7(T) was 32.1 mol%. Taken together, the phenotypic, genotypic and phylogenetic analyses indicate that strain C7(T) represents a novel species of the genus Orbus, for which the name Orbus sasakiae sp. nov. is proposed. The type strain is C7(T) ( = KACC 16544(T) = JCM 18050(T)). An emended description of the genus Orbus is provided.
|
| Volume |
63(Pt 5)
|
| Pages |
1766-1770
|
| Published |
2013-5-1
|
| DOI |
10.1099/ijs.0.041871-0
|
| PMID |
22941300
|
| MeSH |
Animals
Bacterial Typing Techniques
Base Composition
Butterflies / microbiology*
DNA, Bacterial / genetics
Fatty Acids / analysis
Gammaproteobacteria / classification*
Gammaproteobacteria / genetics
Gammaproteobacteria / isolation & purification
Gastrointestinal Tract / microbiology*
Molecular Sequence Data
Phospholipids / analysis
Phylogeny*
RNA, Ribosomal, 16S / genetics
Republic of Korea
Sequence Analysis, DNA
Ubiquinone / analysis
|
| IF |
2.415
|
| Times Cited |
13
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 18050 |
