| RRC ID |
27599
|
| Author |
Saito M, Villanueva SYAM, Kawamura Y, Iida KI, Tomida J, Kanemaru T, Kohno E, Miyahara S, Umeda A, Amako K, Gloriani NG, Yoshida SI.
|
| Title |
Leptospira idonii sp. nov., isolated from environmental water.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Strain Eri-1(T) was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology of the isolate were similar to those of members of the genus Leptospira, but the spiral structure of the isolate was sharper under dark-field microscopy. Cells were 10.6 ± 1.3 µm long and 0.2 µm in diameter, with a wavelength of 0.9 µm and an amplitude of 0.4 µm. Strain Eri-1(T) grew in Korthof's medium at both 13 and 30 °C, and also in the presence of 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain Eri-1(T) within the radiation of the genus Leptospira where it formed a unique lineage within the clade of the known saprophytic species of the genus Leptospira. The strain was not pathogenic to hamsters. Strain Eri-1(T) exhibited low levels (11.2-12.6 %) of similarity by DNA-DNA hybridization to the three most closely related species of the genus Leptospira. The DNA G+C content of the genome of strain Eri-1(T) was 42.5 ± 0.1 mol%. These results suggest that strain Eri-1(T) represents a novel species of the genus Leptospira, for which the name Leptospira idonii sp. nov. is proposed. The type strain is Eri-1(T) ( = DSM 26084(T) = JCM 18486(T)).
|
| Volume |
63(Pt 7)
|
| Pages |
2457-2462
|
| Published |
2013-7-1
|
| DOI |
10.1099/ijs.0.047233-0
|
| PMID |
23203626
|
| MeSH |
Animals
Azaguanine
Bacterial Typing Techniques
Base Composition
Cricetinae
DNA, Bacterial / genetics
Japan
Leptospira / classification*
Leptospira / genetics
Leptospira / isolation & purification
Male
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Water Microbiology*
|
| IF |
2.415
|
| Times Cited |
29
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 18486 |
