| RRC ID |
27622
|
| 著者 |
Gorlas A, Alain K, Bienvenu N, Geslin C.
|
| タイトル |
Thermococcus prieurii sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel hyperthermophilic, anaerobic archaeon, strain Bio-pl-0405IT2(T), was isolated from a hydrothermal chimney sample collected from the East Pacific Rise at 2700 m depth in the 'Sarah Spring' area (7° 25' 24" S 107° 47' 66" W). Cells were irregular, motile cocci (0.8-1.5 µm in diameter) and divided by constriction. Growth was observed at temperatures between 60 °C and 95 °C with an optimum at 80 °C. The pH range for growth was between pH 4.0 and pH 8.0 with an optimum around pH 7.0. Strain Bio-pl-0405IT2(T) grew at salt concentrations of 1-5 % (w/v) NaCl with an optimum at 2 %. The novel isolate grew by fermentation or sulphur respiration on a variety of organic compounds. It was a chemoorganoheterotrophic archaeon growing preferentially with yeast extract, peptone and tryptone as carbon and energy sources and sulphur and organic compounds as electron acceptors; it also grew on maltose and starch. Sulphur or l-cystine were required for growth and were reduced to hydrogen sulfide. The strain was resistant to rifampicin, chloramphenicol, vancomycin and kanamycin (all at 100 µg ml(-1)) but was sensitive to tetracycline. The G+C content of its genomic DNA was 53.6 mol%. Phylogenetic analysis of the almost complete 16S rRNA gene sequence (1450 bp) of strain Bio-pl-0405IT2(T) showed that the novel isolate belonged to the genus Thermococcus. DNA-DNA hybridization values with the two closest relatives Thermococcus hydrothermalis AL662(T) and Thermococcus celer JCM 8558(T) were below the threshold value of 70 %. On the basis of the physiological and genotypic distinctness, we propose a novel species, Thermococcus prieurii sp. nov. The type strain is Bio-pl-0405IT2(T) ( = CSUR P577(T)= JCM 16307(T)).
|
| 巻・号 |
63(Pt 8)
|
| ページ |
2920-2926
|
| 公開日 |
2013-8-1
|
| DOI |
10.1099/ijs.0.026419-0
|
| PMID |
23355695
|
| MeSH |
Base Composition
DNA, Archaeal / genetics
Drug Resistance, Microbial
Hot Temperature
Hydrogen-Ion Concentration
Hydrothermal Vents / microbiology*
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seawater / microbiology
Thermococcus / classification*
Thermococcus / genetics
Thermococcus / growth & development
Water Microbiology
|
| IF |
2.415
|
| 引用数 |
11
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 8558
JCM 10136
JCM 10495
JCM 11827
JCM 16307 |
