論文 - 詳細
| RRC ID | 27630 |
|---|---|
| 著者 | Wagner D, Schirmack J, Ganzert L, Morozova D, Mangelsdorf K. |
| タイトル | Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A methanogenic archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468). |
| 巻・号 | 63(Pt 8) |
| ページ | 2986-2991 |
| 公開日 | 2013-8-1 |
| DOI | 10.1099/ijs.0.046565-0 |
| PMID | 23378113 |
| MeSH | Base Composition DNA, Archaeal / genetics Methanosarcina / classification* Methanosarcina / genetics Methanosarcina / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Siberia Soil Microbiology* |
| IF | 2.415 |
| 引用数 | 36 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18468 |