論文 - 詳細
| RRC ID | 27646 |
|---|---|
| 著者 | Fukuda W, Kimura T, Araki S, Miyoshi Y, Atomi H, Imanaka T. |
| タイトル | Lysobacter oligotrophicus sp. nov., isolated from an Antarctic freshwater lake in Antarctica. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-stain-negative, non-spore-forming, rod-shaped, aerobic bacterium (strain 107-E2(T)) was isolated from freshwater samples containing microbial mats collected at a lake in Skarvsnes, Antarctica (temporary lake name, Lake Tanago Ike). Strain 107-E2(T) grew between 5 and 25 °C, with an optimum of 23 °C. Moreover, colony formation was observed on agar media even at -5 °C. The pH range for growth was between 6.0 and 9.0, with an optimum of pH 7.0-8.0. The range of NaCl concentration for growth was between 0.0 and 0.5% (w/v), with an optimum of 0.0%. No growth was observed in media containing organic compounds at high concentrations, which indicated that strain 107-E2(T) was an oligotroph. In the late stationary phase, strain 107-E2(T) produced a dark brown water-soluble pigment. Esterase, amylase and protease production was observed. Antimicrobial-lytic activities for Gram-negative bacteria and yeast were observed. Ubiquinone-8 was the major respiratory quinone. The major fatty acids were iso-C15:0, iso-C(17:1)ω9c and iso-C(15:1) at 5. The G+C content of genomic DNA was 66.1 mol%. Analysis of the 16S rRNA gene sequences revealed that strain 107-E2(T) belonged to the genus Lysobacter, and low DNA-DNA relatedness values with closely related species distinguished strain 107-E2(T) from recognized species of the genus Lysobacter. The phylogenetic situation and physiological characteristics indicated that strain 107-E2(T) should be classified as a representative of a novel species of the genus Lysobacter, for which the name Lysobacter oligotrophicus sp. nov. is proposed. The type strain is 107-E2(T) ( =JCM 18257(T) =ATCC BAA-2438(T)). |
| 巻・号 | 63(Pt 9) |
| ページ | 3313-3318 |
| 公開日 | 2013-9-1 |
| DOI | 10.1099/ijs.0.051805-0 |
| PMID | 23475347 |
| MeSH | Antarctic Regions Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / analysis Fresh Water / microbiology Lakes / microbiology* Lysobacter / classification* Lysobacter / genetics Lysobacter / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Ubiquinone / analysis |
| IF | 2.415 |
| 引用数 | 28 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18257 |