RRC ID 27663
著者 Vartoukian SR, Downes J, Palmer RM, Wade WG.
タイトル Fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity.
ジャーナル Int J Syst Evol Microbiol
Abstract SGP1(T), a strain belonging to a lineage of the phylum Synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. For good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. Cells of strain SGP1(T) were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth supplemented with a filtered cell sonicate of Fusobacterium nucleatum subsp. nucleatum ATCC 25586(T) (25 %, v/v). Hydrogen sulphide was produced and gelatin was weakly hydrolysed. The major cellular fatty acids were C(14 : 0), C(18 : 0) and C(16 : 0). The DNA G+C content of strain SGP1(T) was 63 mol%. Phylogenetic analysis of the full-length 16S rRNA gene showed that strain SGP1(T) represented a novel group within the phylum Synergistetes. A novel species in a new genus, Fretibacterium fastidiosum gen. nov., sp. nov., is proposed. The type strain of Fretibacterium fastidiosum is SGP1(T) ( = DSM 25557(T) = JCM 16858(T)).
巻・号 63(Pt 2)
ページ 458-463
公開日 2013-2-1
DOI 10.1099/ijs.0.041038-0
PMID 22493171
MeSH Acetic Acid / metabolism Bacteria / classification* Bacteria / genetics Bacteria / isolation & purification Base Composition Coculture Techniques DNA, Bacterial / genetics Fatty Acids / analysis Humans Molecular Sequence Data Mouth / microbiology* Periodontal Pocket / microbiology Phylogeny* Propionates / metabolism RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 32
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16858