RRC ID 2768
Author Horio F, Kiyama K, Kobayashi M, Kawai K, Tsuda T.
Title Ascorbic acid deficiency stimulates hepatic expression of inflammatory chemokine, cytokine-induced neutrophil chemoattractant-1, in scurvy-prone ODS rats.
Journal J Nutr Sci Vitaminol (Tokyo)
Abstract ODS rat has a hereditary defect in ascorbic acid biosynthesis and is a useful animal model for elucidating the physiological role of ascorbic acid. We previously demonstrated by using ODS rats that ascorbic acid deficiency changes the hepatic gene expression of acute phase proteins, as seen in acute inflammation. In this study, we investigated the effects of ascorbic acid deficiency on the production of inflammatory chemokine, cytokine-induced neutrophil chemoattractant-1 (CINC-1), in ODS rats. Male ODS rats (6 wk of age) were fed a basal diet containing ascorbic acid (300 mg/kg diet) or a diet without ascorbic acid for 14 d. Obvious symptoms of scurvy were not observed in the ascorbic acid-deficient rats. Ascorbic acid deficiency significantly elevated the serum concentration of CINC-1 on d 14. The liver and spleen CINC-1 concentrations in the ascorbic acid-deficient rats were significantly elevated to 600% and 180% of the respective values in the control rats. However, the lung concentration of CINC-1 was not affected by ascorbic acid deficiency. Ascorbic acid deficiency significantly elevated the hepatic mRNA level of CINC-1 (to 480% of the value in the control rats), but not the lung mRNA level. These results demonstrate that ascorbic acid deficiency elevates the serum, liver and spleen concentrations of CINC-1 as seen in acute inflammation, and suggest that ascorbic acid deficiency stimulate the hepatic CINC-1 gene expression.
Volume 52(1)
Pages 28-32
Published 2006-2-1
DOI 10.3177/jnsv.52.28
PMID 16637227
MeSH Animals Apolipoprotein A-I / genetics Apolipoproteins E / genetics Ascorbic Acid / analysis Ascorbic Acid / blood Ascorbic Acid Deficiency / genetics Ascorbic Acid Deficiency / metabolism* Blotting, Northern Body Weight Chemokine CXCL1 Chemokines, CXC / analysis Chemokines, CXC / blood Chemokines, CXC / genetics* Gene Expression* Genetic Predisposition to Disease Haptoglobins / genetics Liver / chemistry Liver / metabolism* Lung / chemistry Male Organ Size RNA, Messenger / analysis Rats Rats, Mutant Strains Scurvy / genetics* Spleen / chemistry
IF 1.424
Times Cited 13