論文 - 詳細
| RRC ID | 2770 |
|---|---|
| 著者 | Ikesugi K, Yamamoto R, Mulhern ML, Shinohara T. |
| タイトル | Role of the unfolded protein response (UPR) in cataract formation. |
| ジャーナル | Exp Eye Res |
| Abstract |
Cataract is a multifactorial disease, and a large variety of stressors induce cataracts. Many cataractogenic stressors and endoplasmic reticulum (ER) stressors induce the unfolded protein response (UPR) in various cell types. The UPR is known to produce reactive oxygen species (ROS) prior to the inducement of apoptosis. We investigated whether ER stressors induce the UPR in lens epithelial cells (LECs) or whole rat lenses. Our results showed that higher levels of ER stressors activated Bip/GRP78, ATF4, and caspase-12. In addition, ROS were produced, free glutathione was decreased, and apoptosis was induced. LECs in the mitotic zone were the most susceptible to the UPR while the central LECs were the most resistant. The UPR induced the production of ROS in the ER and probably in the mitochondria. The detectable ROS production in cultured lenses is limited to the epithelial cells. These findings indicate that ER stressors induce the UPR in LECs with and without the induction of apoptosis, and we conclude that the UPR is probably one of the initiating factors of many types of cataracts. |
| 巻・号 | 83(3) |
| ページ | 508-16 |
| 公開日 | 2006-9-1 |
| DOI | 10.1016/j.exer.2006.01.033 |
| PII | S0014-4835(06)00168-0 |
| PMID | 16643900 |
| MeSH | Animals Calcimycin / pharmacology Cataract / metabolism* Cell Line, Transformed Endoplasmic Reticulum Chaperone BiP Epithelial Cells / drug effects Epithelial Cells / metabolism* Glucose / metabolism Glutathione / metabolism Glycosylation / drug effects Homocysteine / pharmacology Humans Ionophores / pharmacology Lens, Crystalline / drug effects Lens, Crystalline / metabolism* Molecular Chaperones Protein Folding* Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism Tissue Culture Techniques Tunicamycin / pharmacology |
| IF | 3.011 |
| 引用数 | 57 |
| WOS 分野 | OPHTHALMOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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