RRC ID 27714
Author Kitajima K, Oki S, Ohkawa Y, Sumi T, Meno C.
Title Wnt signaling regulates left-right axis formation in the node of mouse embryos.
Journal Dev Biol
Abstract The node triggers formation of the left-right axis in mouse embryos by establishing local asymmetry of Nodal and Cerl2 expression. We found that Wnt3 is expressed in perinodal crown cells preferentially on the left side. The enhancer responsible for Wnt3 expression was identified and found to be regulated by Foxa2 and Rbpj under the control of Notch signaling. Rbpj binding sites suppress enhancer activity in pit cells of the node, thereby ensuring crown cell-specific expression. In addition, we found that the expression of Gdf1 and Cerl2 is also regulated by Notch signaling, suggesting that such signaling may induce the expression of genes related to left-right asymmetry as a set. Furthermore, Cerl2 expression became symmetric in response to inhibition of Wnt-β-catenin signaling. Our results suggest that Wnt signaling regulates the asymmetry of Cerl2 expression, which likely generates a left-right difference in Nodal activity at the node for further amplification in lateral plate mesoderm.
Volume 380(2)
Pages 222-32
Published 2013-8-15
DOI 10.1016/j.ydbio.2013.05.011
PII S0012-1606(13)00255-8
PMID 23707899
MeSH Animals Body Patterning* Female Hepatocyte Nuclear Factor 3-beta / physiology Immunoglobulin J Recombination Signal Sequence-Binding Protein / physiology Intercellular Signaling Peptides and Proteins / physiology Mice Mice, Inbred ICR Wnt Signaling Pathway / physiology* Wnt3 Protein / physiology
IF 2.936
Times Cited 19
WOS Category DEVELOPMENTAL BIOLOGY
Resource
DNA material pEF-BOSneo-mNotch1 RAMIC (RDB06771)