RRC ID 27716
Author Mitsui S, Hidaka C, Furihata M, Osako Y, Yuri K.
Title A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6.
Journal Biochem Biophys Res Commun
Abstract A serine protease, motopsin (prss12), plays a significant role in cognitive function and the development of the brain, since the loss of motopsin function causes severe mental retardation in humans and enhances social behavior in mice. Motopsin is activity-dependently secreted from neuronal cells, is captured around the synaptic cleft, and cleaves a proteoglycan, agrin. The multi-domain structure of motopsin, consisting of a signal peptide, a proline-rich domain, a kringle domain, three scavenger receptor cysteine-rich domains, and a protease domain at the C-terminal, suggests the interaction with other molecules through these domains. To identify a protein interacting with motopsin, we performed yeast two-hybrid screening and found that seizure-related gene 6 (sez-6), a transmembrane protein on the plasma membrane of neuronal cells, bound to the proline-rich/kringle domain of motopsin. Pull-down and immunoprecipitation analyses indicated the interaction between these proteins. Immunocytochemical and immunohistochemical analyses suggested the co-localization of motopsin and sez-6 at neuronal cells in the developmental mouse brain and at motor neurons in the anterior horn of human spinal cords. Transient expression of motopsin in neuro2a cells increased the number and length of neurites as well as the level of neurite branching. Interestingly, co-expression of sez-6 with motopsin restored the effect of motopsin at the basal level, while sez-6 expression alone showed no effects on cell morphology. Our results suggest that the interaction of motopsin and sez-6 modulates the neuronal cell morphology.
Volume 436(4)
Pages 638-44
Published 2013-7-12
DOI 10.1016/j.bbrc.2013.04.112
PII S0006-291X(13)00964-9
PMID 23770366
MeSH Animals COS Cells Cells, Cultured Chlorocebus aethiops Intellectual Disability / genetics* Mice Nerve Tissue Proteins / metabolism* Serine Endopeptidases / genetics* Serine Endopeptidases / metabolism
IF 2.705
Times Cited 12
DNA material D-HA (RDB02139) pCALwL (RDB01679)