論文 - 詳細
| RRC ID | 27728 |
|---|---|
| 著者 | Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T. |
| タイトル | Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes. |
| ジャーナル | Sci Rep |
| Abstract |
TAL Effector Nucleases (TALENs) are versatile tools for targeted gene editing in various species. However, their efficiency is still insufficient, especially in mammalian embryos. Here, we showed that combined expression of Exonuclease 1 (Exo1) with engineered site-specific TALENs provided highly efficient disruption of the endogenous gene in rat fibroblast cells. A similar increased efficiency of up to ~30% with Exo1 was also observed in fertilized rat eggs, and in the production of knockout rats for the albino (Tyr) gene. These findings demonstrate TALENs with Exo1 is an easy and efficient method of generating gene knockouts using zygotes, which increases the range of gene targeting technologies available to various species. |
| 巻・号 | 3 |
| ページ | 1253 |
| 公開日 | 2013-2-13 |
| DOI | 10.1038/srep01253 |
| PII | srep01253 |
| PMID | 23409244 |
| PMC | PMC3570776 |
| MeSH | Animals Base Sequence Cell Line Endonucleases / genetics* Endonucleases / metabolism Exodeoxyribonucleases / genetics* Exodeoxyribonucleases / metabolism Female Gene Knockout Techniques Gene Targeting* HEK293 Cells Humans Monophenol Monooxygenase / genetics Plasmids / genetics Plasmids / metabolism RNA, Messenger / metabolism Rats Transfection Zygote / metabolism* |
| IF | 3.998 |
| 引用数 | 54 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | DA-Tyrem1Kyo(strainID=1092) DA-Tyrem2Kyo(strainID=1093) |
| ヒト・動物細胞 | Rat-1(RCB1830) |