RRC ID 27741
Author Kodama A, Kamei N, Kamei G, Kongcharoensombat W, Ohkawa S, Nakabayashi A, Ochi M.
Title In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model.
Journal J Bone Joint Surg Br
Abstract For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.
Volume 94(7)
Pages 998-1006
Published 2012-7
DOI 10.1302/0301-620X.94B7.28521
PII 94-B/7/998
PMID 22733960
MeSH Animals Bone Regeneration / physiology Cell Differentiation / physiology Cell Movement Dextrans Disease Models, Animal Female Femoral Fractures / pathology Femoral Fractures / physiopathology Femoral Fractures / therapy* Fracture Healing / physiology* Fractures, Ununited / pathology Fractures, Ununited / physiopathology Fractures, Ununited / therapy* Luminescent Measurements / methods Magnetic Fields* Magnetite Nanoparticles Mesenchymal Stem Cell Transplantation* Mesenchymal Stromal Cells / pathology Mesenchymal Stromal Cells / physiology Rats Rats, Inbred Lew
Times Cited 17
Rats LEW-Tg(Gt(ROSA)26Sor-luc)11Jmsk(strainID=649) LEW-Tg(Gt(ROSA)26Sor-lacZ)44Jmsk(strainID=648)