RRC ID 2777
著者 Mashimo T, Voigt B, Tsurumi T, Naoi K, Nakanishi S, Yamasaki K, Kuramoto T, Serikawa T.
タイトル A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains.
ジャーナル BMC Genet
Abstract BACKGROUND:The National Bio Resource Project for the Rat in Japan (NBRP-Rat) is focusing on collecting, preserving and distributing various rat strains, including spontaneous mutant, transgenic, congenic, and recombinant inbred (RI) strains. To evaluate their value as models of human diseases, we are characterizing them using 109 phenotypic parameters, such as clinical measurements, internal anatomy, metabolic parameters, and behavioral tests, as part of the Rat Phenome Project. Here, we report on a set of 357 simple sequence length polymorphism (SSLP) markers and 122 rat strains, which were genotyped by the marker set.
RESULTS:The SSLP markers were selected according to their distribution patterns throughout the whole rat genome with an average spacing of 7.59 Mb. The average number of informative markers between all possible pairs of strains was 259 (72.5% of 357 markers), showing their high degree of polymorphism. From the genetic profile of these rat inbred strains, we constructed a rat family tree to clarify their genetic background.
CONCLUSION:These highly informative SSLP markers as well as genetically and phenotypically defined rat strains are useful for designing experiments for quantitative trait loci (QTL) analysis and to choose strategies for developing new genetic resources. The data and resources are freely available at the NBRP-Rat web site 1.
巻・号 7
ページ 19
公開日 2006-4-4
DOI 10.1186/1471-2156-7-19
PII 1471-2156-7-19
PMID 16584579
PMC PMC1475628
MeSH Animals Animals, Congenic / genetics Animals, Genetically Modified / genetics Breeding Crosses, Genetic Female Genetic Markers Genome Genotype Japan Male Phenotype Phylogeny Polymorphism, Genetic / genetics* Rats Rats, Inbred Strains / classification Rats, Inbred Strains / genetics* Rats, Mutant Strains / classification Rats, Mutant Strains / genetics* Specific Pathogen-Free Organisms
IF 2.567
引用数 28
WOS 分野 GENETICS & HEREDITY
リソース情報
ラット 122 strains