論文 - 詳細
| RRC ID | 27773 |
|---|---|
| 著者 | Ysebrant de Lendonck L, Tonon S, Nguyen M, Vandevenne P, Welsby I, Martinet V, Molle C, Charbonnier LM, Leo O, Goriely S. |
| タイトル | Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
IFN regulatory factor (IRF) 3 plays a key role in innate responses against viruses. Herein we assessed its contribution to T-cell activation. We observed that poly(I:C)-induced IRF3 activation in CD8 T cells represses IL-17 expression in a type I IFN-independent fashion. Even in the absence of poly(I:C), polyclonally activated naïve IRF3(-/-) CD8 T cells expressed high levels of IL-17 and IL-23R in comparison with wild-type cells. Furthermore, IRF3(-/-) OT1 cells adoptively transferred into wild-type hosts also produced higher IL-17 levels upon immunization than their wild-type counterparts. This phenotype could be reversed by ectopic expression of IRF3, confirming that this effect is intrinsic to T cells. We show that IRF3 directly interacts with RORγt in the cytoplasm through its IRF interaction domain and limits its ability to bind and transactivate the IL-17 promoter. These observations uncover an unexpected role of IRF3 in the control of CD8 T-cell polarization. |
| 巻・号 | 110(34) |
| ページ | E3189-97 |
| 公開日 | 2013-8-20 |
| DOI | 10.1073/pnas.1219221110 |
| PII | 1219221110 |
| PMID | 23918362 |
| PMC | PMC3752229 |
| MeSH | Adaptive Immunity / immunology* Animals Blotting, Western CD8-Positive T-Lymphocytes / metabolism* Chromatin Immunoprecipitation Enzyme-Linked Immunosorbent Assay Gene Expression Regulation / immunology* HEK293 Cells Humans Immunity, Innate / immunology* Interferon Regulatory Factor-3 / metabolism* Interleukin-17 / metabolism* Luciferases Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Fluorescence Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction |
| IF | 9.412 |
| 引用数 | 17 |
| WOS 分野 | IMMUNOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC00858 |