RRC ID |
27883
|
著者 |
Tian F, Morimoto N, Liu W, Ohta Y, Deguchi K, Miyazaki K, Abe K.
|
タイトル |
In vivo optical imaging of motor neuron autophagy in a mouse model of amyotrophic lateral sclerosis.
|
ジャーナル |
Autophagy
|
Abstract |
Autophagy is involved in the pathological process of motor neuron death in amyotrophic lateral sclerosis (ALS). We have generated a novel double transgenic (DTg) mouse line by mating a green fluorescent protein (GFP)-fused microtubule-associated protein 1 light chain 3 (LC3) transgenic (LC3-Tg) mouse and a G93A mutant human Cu/Zn superoxide dismutase (mSOD1) transgenic (mSOD1-Tg) mouse. In vivo imaging of autophagy with these novel DTg mice was conducted at 10 (presymptomatic), 17 (early symptomatic) and 19 (late symptomatic) weeks of age. Fluorescence imaging analysis revealed a strong fluorescent signal in vivo over the T₃-S₁ level at 17 and 19 weeks of age only in the DTg mice. Ex vivo autophagy imaging of spinal cord sections (20 μm) also showed a progressive increase of the fluorescence signal from 17 to 19 weeks in DTg mice in the anterior horn at the L₄-₅ level, and the fluorescence signals were clearly observed in the gray matter of the spinal cord with a progressive increase of the signal and decreases in large motor neurons. Protein gel blot analysis revealed maximum LC3-I and LC3-II expressions at 19 weeks, consistent with the results from the in vivo autophagy imaging experiment. This method could also be applied as a unique tool for clarifying the role of autophagy, and to monitor the pathologic processes involving autophagy not only in ALS, but also other neurological diseases.
|
巻・号 |
7(9)
|
ページ |
985-92
|
公開日 |
2011-9-1
|
DOI |
10.4161/auto.7.9.16012
|
PII |
16012
|
PMID |
21628996
|
MeSH |
Amyotrophic Lateral Sclerosis / diagnosis*
Amyotrophic Lateral Sclerosis / pathology*
Animals
Astrocytes / metabolism
Astrocytes / pathology
Autophagy*
Cell Count
Diagnostic Imaging / methods*
Disease Models, Animal
Fluorescent Antibody Technique
Green Fluorescent Proteins / metabolism
Humans
Mice
Mice, Transgenic
Microtubule-Associated Proteins / metabolism
Motor Neurons / pathology*
Optical Phenomena*
Phagosomes / metabolism
Recombinant Fusion Proteins / metabolism
|
IF |
9.77
|
引用数 |
37
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00806 |