論文 - 詳細
| RRC ID | 27883 |
|---|---|
| 著者 | Tian F, Morimoto N, Liu W, Ohta Y, Deguchi K, Miyazaki K, Abe K. |
| タイトル | In vivo optical imaging of motor neuron autophagy in a mouse model of amyotrophic lateral sclerosis. |
| ジャーナル | Autophagy |
| Abstract |
Autophagy is involved in the pathological process of motor neuron death in amyotrophic lateral sclerosis (ALS). We have generated a novel double transgenic (DTg) mouse line by mating a green fluorescent protein (GFP)-fused microtubule-associated protein 1 light chain 3 (LC3) transgenic (LC3-Tg) mouse and a G93A mutant human Cu/Zn superoxide dismutase (mSOD1) transgenic (mSOD1-Tg) mouse. In vivo imaging of autophagy with these novel DTg mice was conducted at 10 (presymptomatic), 17 (early symptomatic) and 19 (late symptomatic) weeks of age. Fluorescence imaging analysis revealed a strong fluorescent signal in vivo over the T₃-S₁ level at 17 and 19 weeks of age only in the DTg mice. Ex vivo autophagy imaging of spinal cord sections (20 μm) also showed a progressive increase of the fluorescence signal from 17 to 19 weeks in DTg mice in the anterior horn at the L₄-₅ level, and the fluorescence signals were clearly observed in the gray matter of the spinal cord with a progressive increase of the signal and decreases in large motor neurons. Protein gel blot analysis revealed maximum LC3-I and LC3-II expressions at 19 weeks, consistent with the results from the in vivo autophagy imaging experiment. This method could also be applied as a unique tool for clarifying the role of autophagy, and to monitor the pathologic processes involving autophagy not only in ALS, but also other neurological diseases. |
| 巻・号 | 7(9) |
| ページ | 985-92 |
| 公開日 | 2011-9-1 |
| DOI | 10.4161/auto.7.9.16012 |
| PII | 16012 |
| PMID | 21628996 |
| MeSH | Amyotrophic Lateral Sclerosis / diagnosis* Amyotrophic Lateral Sclerosis / pathology* Animals Astrocytes / metabolism Astrocytes / pathology Autophagy* Cell Count Diagnostic Imaging / methods* Disease Models, Animal Fluorescent Antibody Technique Green Fluorescent Proteins / metabolism Humans Mice Mice, Transgenic Microtubule-Associated Proteins / metabolism Motor Neurons / pathology* Optical Phenomena* Phagosomes / metabolism Recombinant Fusion Proteins / metabolism |
| IF | 9.77 |
| 引用数 | 37 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |