| RRC ID |
27912
|
| Author |
Fontrodona L, Porta-de-la-Riva M, Morán T, Niu W, Díaz M, Aristizábal-Corrales D, Villanueva A, Schwartz S Jr, Reinke V, Cerón J.
|
| Title |
RSR-2, the Caenorhabditis elegans ortholog of human spliceosomal component SRm300/SRRM2, regulates development by influencing the transcriptional machinery.
|
| Journal |
PLoS Genet
|
| Abstract |
Protein components of the spliceosome are highly conserved in eukaryotes and can influence several steps of the gene expression process. RSR-2, the Caenorhabditis elegans ortholog of the human spliceosomal protein SRm300/SRRM2, is essential for viability, in contrast to the yeast ortholog Cwc21p. We took advantage of mutants and RNA interference (RNAi) to study rsr-2 functions in C. elegans, and through genetic epistasis analysis found that rsr-2 is within the germline sex determination pathway. Intriguingly, transcriptome analyses of rsr-2(RNAi) animals did not reveal appreciable splicing defects but instead a slight global decrease in transcript levels. We further investigated this effect in transcription and observed that RSR-2 colocalizes with DNA in germline nuclei and coprecipitates with chromatin, displaying a ChIP-Seq profile similar to that obtained for the RNA Polymerase II (RNAPII). Consistent with a novel transcription function we demonstrate that the recruitment of RSR-2 to chromatin is splicing-independent and that RSR-2 interacts with RNAPII and affects RNAPII phosphorylation states. Proteomic analyses identified proteins associated with RSR-2 that are involved in different gene expression steps, including RNA metabolism and transcription with PRP-8 and PRP-19 being the strongest interacting partners. PRP-8 is a core component of the spliceosome and PRP-19 is the core component of the PRP19 complex, which interacts with RNAPII and is necessary for full transcriptional activity. Taken together, our study proposes that RSR-2 is a multifunctional protein whose role in transcription influences C. elegans development.
|
| Volume |
9(6)
|
| Pages |
e1003543
|
| Published |
2013-6-1
|
| DOI |
10.1371/journal.pgen.1003543
|
| PII |
PGENETICS-D-12-02352
|
| PMID |
23754964
|
| PMC |
PMC3675011
|
| MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / growth & development
Caenorhabditis elegans Proteins / genetics*
Chromatin / genetics*
DNA-Binding Proteins / genetics*
Germ Cells
Humans
Phosphorylation
RNA Polymerase II
RNA Splicing / genetics
RNA-Binding Proteins / genetics
Sequence Homology, Amino Acid
Spliceosomes / genetics*
Spliceosomes / metabolism
Transcription, Genetic*
|
| IF |
5.175
|
| Times Cited |
9
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| C.elegans |
tm2625
tm2607 |
