論文 - 詳細
| RRC ID | 27921 |
|---|---|
| 著者 | Jakociunas T, Domange Jordö M, Aït Mebarek M, Bünner CM, Verhein-Hansen J, Oddershede LB, Thon G. |
| タイトル | Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Our research addresses the relationship between subnuclear localization and gene expression in fission yeast. We observed the relocalization of a heterochromatic region, the mating-type region, from its natural location at the spindle-pole body to the immediate vicinity of the nucleolus. Relocalization occurred in response to a DNA rearrangement replacing a boundary element (IR-R) with a ribosomal DNA repeat (rDNA-R). Gene expression was strongly silenced in the relocalized mating-type region through mechanisms that differ from those operating in wild type. Also different from the wild-type situation, programmed recombination events failed to take place in the rDNA-R mutant. Increased silencing and perinucleolar localization depended on Reb1, a DNA-binding protein with cognate sites in the rDNA. Reb1 was recently shown to mediate long-range interchromosomal interactions in the nucleus through dimerization, providing a mechanism for the observed relocalization. Replacing the full rDNA repeat with Reb1-binding sites, and using mutants lacking the histone H3K9 methyltransferase Clr4, indicated that the relocalized region was silenced redundantly by heterochromatin and another mechanism, plausibly antisense transcription, achieving a high degree of repression in the rDNA-R strain. |
| 巻・号 | 110(47) |
| ページ | E4465-73 |
| 公開日 | 2013-11-19 |
| DOI | 10.1073/pnas.1315581110 |
| PII | 1315581110 |
| PMID | 24191010 |
| PMC | PMC3839722 |
| MeSH | Binding Sites / genetics DNA, Ribosomal / genetics* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Silencing* Heterochromatin / physiology* Intranuclear Space / metabolism* Multiplex Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid / genetics Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 9.412 |
| 引用数 | 12 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | S. pombe YFP-FLAG-His SpYFH08C03 (SPW083251) |
| 酵母 | |