RRC ID 27921
Author Jakociunas T, Domange Jordö M, Aït Mebarek M, Bünner CM, Verhein-Hansen J, Oddershede LB, Thon G.
Title Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat.
Journal Proc Natl Acad Sci U S A
Abstract Our research addresses the relationship between subnuclear localization and gene expression in fission yeast. We observed the relocalization of a heterochromatic region, the mating-type region, from its natural location at the spindle-pole body to the immediate vicinity of the nucleolus. Relocalization occurred in response to a DNA rearrangement replacing a boundary element (IR-R) with a ribosomal DNA repeat (rDNA-R). Gene expression was strongly silenced in the relocalized mating-type region through mechanisms that differ from those operating in wild type. Also different from the wild-type situation, programmed recombination events failed to take place in the rDNA-R mutant. Increased silencing and perinucleolar localization depended on Reb1, a DNA-binding protein with cognate sites in the rDNA. Reb1 was recently shown to mediate long-range interchromosomal interactions in the nucleus through dimerization, providing a mechanism for the observed relocalization. Replacing the full rDNA repeat with Reb1-binding sites, and using mutants lacking the histone H3K9 methyltransferase Clr4, indicated that the relocalized region was silenced redundantly by heterochromatin and another mechanism, plausibly antisense transcription, achieving a high degree of repression in the rDNA-R strain.
Volume 110(47)
Pages E4465-73
Published 2013-11-19
DOI 10.1073/pnas.1315581110
PII 1315581110
PMID 24191010
PMC PMC3839722
MeSH Binding Sites / genetics DNA, Ribosomal / genetics* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Silencing* Heterochromatin / physiology* Intranuclear Space / metabolism* Multiplex Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid / genetics Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 9.412
Times Cited 12
DNA material S. pombe YFP-FLAG-His SpYFH08C03 (SPW083251)