RRC ID 27921
Author Jakociunas T, Domange Jordö M, Aït Mebarek M, Bünner CM, Verhein-Hansen J, Oddershede LB, Thon G.
Title Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Our research addresses the relationship between subnuclear localization and gene expression in fission yeast. We observed the relocalization of a heterochromatic region, the mating-type region, from its natural location at the spindle-pole body to the immediate vicinity of the nucleolus. Relocalization occurred in response to a DNA rearrangement replacing a boundary element (IR-R) with a ribosomal DNA repeat (rDNA-R). Gene expression was strongly silenced in the relocalized mating-type region through mechanisms that differ from those operating in wild type. Also different from the wild-type situation, programmed recombination events failed to take place in the rDNA-R mutant. Increased silencing and perinucleolar localization depended on Reb1, a DNA-binding protein with cognate sites in the rDNA. Reb1 was recently shown to mediate long-range interchromosomal interactions in the nucleus through dimerization, providing a mechanism for the observed relocalization. Replacing the full rDNA repeat with Reb1-binding sites, and using mutants lacking the histone H3K9 methyltransferase Clr4, indicated that the relocalized region was silenced redundantly by heterochromatin and another mechanism, plausibly antisense transcription, achieving a high degree of repression in the rDNA-R strain.
Volume 110(47)
Pages E4465-73
Published 2013-11-19
DOI 10.1073/pnas.1315581110
PII 1315581110
PMID 24191010
PMC PMC3839722
MeSH Binding Sites / genetics DNA, Ribosomal / genetics* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Silencing* Heterochromatin / physiology* Intranuclear Space / metabolism* Multiplex Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid / genetics Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 9.504
Times Cited 7
DNA material S. pombe YFP-FLAG-His SpYFH08C03 (SPW083251)