Reference - Detail
| RRC ID | 2796 |
|---|---|
| Author | Takahashi M, Haruta M. |
| Title | Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro. |
| Journal | Methods Mol Biol |
| Abstract |
For cell replacement therapies of retinal diseases, the most-needed cells are retinal pigment epithelial cells and photoreceptor cells; lens cells are needed for replacement of the cataract. Stromal cell-derived inducing activity induces the differentiation of mouse embryonic stem (ES) cells into neural cells, including midbrain tyrosine hydroxylase-positive dopaminergic neurons, and ocular cells; the method also works with primate ES cells. We describe the methods to induce retinal pigment epithelial and lens cells from monkey ES cells using stromal cell-derived inducing activity and show the characteristics of those cells in vitro and in vivo. |
| Volume | 330 |
| Pages | 417-29 |
| Published | 2006-1-1 |
| DOI | 10.1385/1-59745-036-7:417 |
| PMID | 16846040 |
| MeSH | Animals Behavior, Animal / physiology Cell Culture Techniques* Cell Differentiation / physiology* Cell Transplantation Cells, Cultured Embryo, Mammalian / cytology Epithelial Cells / cytology Epithelial Cells / physiology* Macaca fascicularis Mice Pigment Epithelium of Eye / cytology* Rats Stem Cells / cytology Stem Cells / physiology* |
| Resource | |
| Rats | |
| Human and Animal Cells | STO(RCB0536) OP9(RCB1124) MC3T3-G2/PA6(RCB1127) |