Reference - Detail
| RRC ID | 28072 |
|---|---|
| Author | Neumüller RA, Gross T, Samsonova AA, Vinayagam A, Buckner M, Founk K, Hu Y, Sharifpoor S, Rosebrock AP, Andrews B, Winston F, Perrimon N. |
| Title | Conserved regulators of nucleolar size revealed by global phenotypic analyses. |
| Journal | Sci Signal |
| Abstract |
Regulation of cell growth is a fundamental process in development and disease that integrates a vast array of extra- and intracellular information. A central player in this process is RNA polymerase I (Pol I), which transcribes ribosomal RNA (rRNA) genes in the nucleolus. Rapidly growing cancer cells are characterized by increased Pol I-mediated transcription and, consequently, nucleolar hypertrophy. To map the genetic network underlying the regulation of nucleolar size and of Pol I-mediated transcription, we performed comparative, genome-wide loss-of-function analyses of nucleolar size in Saccharomyces cerevisiae and Drosophila melanogaster coupled with mass spectrometry-based analyses of the ribosomal DNA (rDNA) promoter. With this approach, we identified a set of conserved and nonconserved molecular complexes that control nucleolar size. Furthermore, we characterized a direct role of the histone information regulator (HIR) complex in repressing rRNA transcription in yeast. Our study provides a full-genome, cross-species analysis of a nuclear subcompartment and shows that this approach can identify conserved molecular modules. |
| Volume | 6(289) |
| Pages | ra70 |
| Published | 2013-8-20 |
| DOI | 10.1126/scisignal.2004145 |
| PII | 6/289/ra70 |
| PMID | 23962978 |
| PMC | PMC3964804 |
| MeSH | Cell Nucleolus / genetics Cell Nucleolus / metabolism* DNA, Fungal / genetics DNA, Fungal / metabolism DNA, Ribosomal / genetics DNA, Ribosomal / metabolism Genes, Fungal / physiology Genes, rRNA / physiology Histones / genetics Histones / metabolism RNA Polymerase I / genetics RNA Polymerase I / metabolism* RNA, Fungal / biosynthesis* RNA, Fungal / genetics RNA, Ribosomal / biosynthesis* RNA, Ribosomal / genetics Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Transcription, Genetic / physiology* |
| IF | 6.467 |
| Times Cited | 39 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 17 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 1828R-2 1828R-3 4817R-2 |