Reference - Detail
| RRC ID | 28090 |
|---|---|
| Author | Oh-Hashi K, Hirata Y, Kiuchi K. |
| Title | Transcriptional regulation of mouse mesencephalic astrocyte-derived neurotrophic factor in Neuro2a cells. |
| Journal | Cell Mol Biol Lett |
| Abstract |
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a novel type of trophic factor. Recent studies indicate that the MANF gene is induced in response to endoplasmic reticulum (ER) stress through ER stress response element II (ERSE-II) in its 5'-flanking region. In this study, we evaluated the roles of six ER stress response transcription factors in the regulation of the promoter activities of the mouse MANF gene via ERSE-II using various types of mutant MANF luciferase reporter constructs. Treatment with thapsigargin (Tg) induced MANF mRNA generation in parallel with the elevation of ATF6α, sXBP and Luman mRNA levels in Neuro2a cells. Of the six transcription factors, ATF6β most strongly increased the MANF promoter activity via ERSE-II, while the effects of ATF6β and sXBP1 were moderate. However, overexpression of Luman or OASIS did not enhance ERSE-II-dependent MANF promoter activity in Neuro2a cells. To evaluate the relationships between transcription factors in the regulation of ERSE-II-dependent MANF promoter activity, we transfected two effective transcription factor constructs chosen from ATF6α, ATF6β, uXBP1 and sXBP1 into Neuro2a cells with the MANF reporter construct. The MANF promoter activity induced by co-transfection of ATF6α with ATF6β was significantly lower than that induced by ATF6α alone, while other combinations did not show any effect on the ERSE-II-dependent MANF promoter activity in Neuro2a cells. Our study is the first to show the efficiency of ER stress-related transcription factors for ERSE-II in activating the transcription of the mouse MANF gene in Neuro2a cells. |
| Volume | 18(3) |
| Pages | 398-415 |
| Published | 2013-9-1 |
| DOI | 10.2478/s11658-013-0096-x |
| PMID | 23864333 |
| PMC | PMC6275609 |
| MeSH | Activating Transcription Factor 6 / genetics Activating Transcription Factor 6 / metabolism Animals Blotting, Western Cell Line, Tumor DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Endoplasmic Reticulum Stress / genetics Enzyme Inhibitors / pharmacology Gene Expression Regulation / drug effects Luciferases / genetics Luciferases / metabolism Mice Mutation* Nerve Growth Factors / genetics* Neuroblastoma / genetics Neuroblastoma / metabolism Neuroblastoma / pathology Promoter Regions, Genetic / genetics* Regulatory Factor X Transcription Factors Reverse Transcriptase Polymerase Chain Reaction Thapsigargin / pharmacology Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic* Transfection |
| IF | 3.451 |
| Times Cited | 15 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pCMFlag_hsCREB3L1 (RDB06267) |