RRC ID 28096
Author Ibrahim DM, Biehs B, Kornberg TB, Klebes A.
Title Microarray comparison of anterior and posterior Drosophila wing imaginal disc cells identifies novel wing genes.
Journal G3 (Bethesda)
Abstract Signaling between cells in the anterior (A) and posterior (P) compartments directs Drosophila wing disc development and is dependent on expression of the homeodomain transcription factor Engrailed (En) in P cells. Downstream of en, posteriorly expressed Hedgehog (Hh) protein signals across the A/P border to establish a developmental organizer that directs pattern formation and growth throughout the wing primordium. Here we extend investigations of the processes downstream of en by using expression array analysis to compare A and P cells. A total of 102 candidate genes were identified that express differentially in the A and P compartments; four were characterized: Stubble (Sb) expression is restricted to A cells due to repression by en. CG15905, CG16884; CG10200/hase und igel (hui) are expressed in A cells downstream of Hh signaling; and RNA interference for hui, Stubble, and CG16884 revealed that each is essential to wing development.
Volume 3(8)
Pages 1353-62
Published 2013-8-7
DOI 10.1534/g3.113.006569
PII g3.113.006569
PMID 23749451
PMC PMC3737175
MeSH Animals Chromosome Mapping Drosophila / genetics* Drosophila / growth & development Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / genetics* Drosophila Proteins / metabolism Gene Expression Regulation, Developmental Hedgehog Proteins / genetics Hedgehog Proteins / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Imaginal Discs / cytology* Imaginal Discs / metabolism Membrane Proteins / antagonists & inhibitors Membrane Proteins / genetics Membrane Proteins / metabolism Oligonucleotide Array Sequence Analysis RNA Interference Serine Endopeptidases / genetics Serine Endopeptidases / metabolism Signal Transduction / genetics Transcription Factors / genetics Transcription Factors / metabolism Wings, Animal / cytology*
IF 2.742
Times Cited 5
Drosophila 15905R-1 16884R-2 4316R-1