RRC ID 281
著者 Leulier F, Vidal S, Saigo K, Ueda R, Lemaitre B.
タイトル Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults.
ジャーナル Curr Biol
Abstract In Drosophila, the immune deficiency (Imd) pathway controls antibacterial peptide gene expression in the fat body in response to Gram-negative bacterial infection. The ultimate target of the Imd pathway is Relish, a transactivator related to mammalian P105 and P100 NF-kappaB precursors. Relish is processed in order to translocate to the nucleus, and this cleavage is dependent on both Dredd, an apical caspase related to caspase-8 of mammals, and the fly Ikappa-B kinase complex (dmIKK). dTAK1, a MAPKKK, functions upstream of the dmIKK complex and downstream of Imd, a protein with a death domain similar to that of mammalian receptor interacting protein (RIP). Finally, the peptidoglycan recognition protein-LC (PGRP-LC) acts upstream of Imd and probably functions as a receptor for the Imd pathway. Using inducible expression of dFADD double-stranded RNA, we demonstrate that dFADD is a novel component of the Imd pathway: dFADD double-stranded RNA expression reduces the induction of antibacterial peptide-encoding genes after infection and renders the fly susceptible to Gram-negative bacterial infection. Epistatic studies indicate that dFADD acts between Imd and Dredd. Our results reinforce the parallels between the Imd and the TNF-R1 pathways.
巻・号 12(12)
ページ 996-1000
公開日 2002-6-25
DOI 10.1016/s0960-9822(02)00873-4
PII S0960-9822(02)00873-4
PMID 12123572
MeSH Adaptor Proteins, Signal Transducing* Animals Animals, Genetically Modified Carrier Proteins / genetics Carrier Proteins / physiology* Drosophila / genetics Drosophila / immunology Drosophila / microbiology* Drosophila Proteins / genetics Drosophila Proteins / physiology Fas-Associated Death Domain Protein Female Gene Expression Regulation Gram-Negative Bacteria / physiology Insect Proteins / genetics Male RNA, Double-Stranded / biosynthesis
IF 9.601
引用数 136
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ 1388R-1 1388R-2 12297R-1 12297R-2 GFP-IR-1