| RRC ID |
28108
|
| 著者 |
Sugiyama T, Ishida T, Tabei N, Shigyo M, Konishi M, Yoneyama T, Yanagisawa S.
|
| タイトル |
Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments in Physcomitrella patens.
|
| ジャーナル |
J Exp Bot
|
| Abstract |
In higher plants, the Dof transcription factors that harbour a conserved plant-specific DNA-binding domain function in the regulation of diverse biological processes that are unique to plants. Although these factors are present in both higher and lower plants, they have not yet been characterized in lower plants. Here six genes encoding Dof transcription factors in the moss Physcomitrella patens are characterized and two of these genes, PpDof1 and PpDof2, are functionally analysed. The targeted disruption of PpDof1 caused delayed or reduced gametophore formation, accompanied by an effect on development of the caulonema from the chloronema. Furthermore, the ppdof1 disruptants were found to form smaller colonies with a reduced frequency of branching of protonemal filaments, depending on the nutrients in the media. Most of these phenotypes were not apparent in the ppdof2 disruptant, although the ppdof2 disruptants also formed smaller colonies on a particular medium. Transcriptional repressor activity of PpDof1 and PpDof2 and modified expression of a number of genes in the ppdof disruptant lines were also shown. These results thus suggest that the PpDof1 transcriptional repressor has a role in controlling nutrient-dependent filament growth.
|
| 巻・号 |
63(8)
|
| ページ |
3185-97
|
| 公開日 |
2012-5-1
|
| DOI |
10.1093/jxb/ers042
|
| PII |
ers042
|
| PMID |
22345635
|
| PMC |
PMC3350930
|
| MeSH |
Amino Acid Sequence
Bryopsida / cytology*
Bryopsida / drug effects
Bryopsida / genetics
Bryopsida / growth & development*
Culture Media / pharmacology*
Gene Expression Regulation, Plant / drug effects
Genes, Plant / genetics
Germ Cells, Plant / cytology
Germ Cells, Plant / drug effects
Germ Cells, Plant / metabolism
Molecular Sequence Data
Mutation / genetics
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Repressor Proteins / chemistry
Repressor Proteins / genetics
Repressor Proteins / metabolism*
Transcription, Genetic* / drug effects
|
| IF |
5.908
|
| 引用数 |
18
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pdp31562
pdp36135
pdp02416
pdp17798
pdp13251 |
