| RRC ID |
28115
|
| 著者 |
Cheng Y, Zhou Y, Yang Y, Chi YJ, Zhou J, Chen JY, Wang F, Fan B, Shi K, Zhou YH, Yu JQ, Chen Z.
|
| タイトル |
Structural and functional analysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors.
|
| ジャーナル |
Plant Physiol
|
| Abstract |
WRKY transcription factors are encoded by a large gene superfamily with a broad range of roles in plants. Recently, several groups have reported that proteins containing a short VQ (FxxxVQxLTG) motif interact with WRKY proteins. We have recently discovered that two VQ proteins from Arabidopsis (Arabidopsis thaliana), SIGMA FACTOR-INTERACTING PROTEIN1 and SIGMA FACTOR-INTERACTING PROTEIN2, act as coactivators of WRKY33 in plant defense by specifically recognizing the C-terminal WRKY domain and stimulating the DNA-binding activity of WRKY33. In this study, we have analyzed the entire family of 34 structurally divergent VQ proteins from Arabidopsis. Yeast (Saccharomyces cerevisiae) two-hybrid assays showed that Arabidopsis VQ proteins interacted specifically with the C-terminal WRKY domains of group I and the sole WRKY domains of group IIc WRKY proteins. Using site-directed mutagenesis, we identified structural features of these two closely related groups of WRKY domains that are critical for interaction with VQ proteins. Quantitative reverse transcription polymerase chain reaction revealed that expression of a majority of Arabidopsis VQ genes was responsive to pathogen infection and salicylic acid treatment. Functional analysis using both knockout mutants and overexpression lines revealed strong phenotypes in growth, development, and susceptibility to pathogen infection. Altered phenotypes were substantially enhanced through cooverexpression of genes encoding interacting VQ and WRKY proteins. These findings indicate that VQ proteins play an important role in plant growth, development, and response to environmental conditions, most likely by acting as cofactors of group I and IIc WRKY transcription factors.
|
| 巻・号 |
159(2)
|
| ページ |
810-25
|
| 公開日 |
2012-6-1
|
| DOI |
10.1104/pp.112.196816
|
| PII |
pp.112.196816
|
| PMID |
22535423
|
| PMC |
PMC3375943
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Botrytis / pathogenicity
DNA, Plant / genetics
DNA-Binding Proteins / metabolism
Disease Resistance
Gene Expression Regulation, Plant
Genes, Plant
Molecular Sequence Data
Mutagenesis, Site-Directed
Phenotype
Phylogeny
Plant Diseases / immunology
Plant Immunity
Plant Leaves / genetics
Plant Leaves / metabolism
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Promoter Regions, Genetic
Protein Interaction Mapping*
Pseudomonas syringae / pathogenicity
Salicylic Acid / pharmacology
Sigma Factor / genetics
Sigma Factor / metabolism
Structure-Activity Relationship
Substrate Specificity
Transcription Factors / genetics
Transcription Factors / metabolism*
Two-Hybrid System Techniques
|
| IF |
6.902
|
| 引用数 |
107
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pst10287
pst02467
pst13186
pst20793 |
