Reference - Detail
| RRC ID | 28115 |
|---|---|
| Author | Cheng Y, Zhou Y, Yang Y, Chi YJ, Zhou J, Chen JY, Wang F, Fan B, Shi K, Zhou YH, Yu JQ, Chen Z. |
| Title | Structural and functional analysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors. |
| Journal | Plant Physiol |
| Abstract |
WRKY transcription factors are encoded by a large gene superfamily with a broad range of roles in plants. Recently, several groups have reported that proteins containing a short VQ (FxxxVQxLTG) motif interact with WRKY proteins. We have recently discovered that two VQ proteins from Arabidopsis (Arabidopsis thaliana), SIGMA FACTOR-INTERACTING PROTEIN1 and SIGMA FACTOR-INTERACTING PROTEIN2, act as coactivators of WRKY33 in plant defense by specifically recognizing the C-terminal WRKY domain and stimulating the DNA-binding activity of WRKY33. In this study, we have analyzed the entire family of 34 structurally divergent VQ proteins from Arabidopsis. Yeast (Saccharomyces cerevisiae) two-hybrid assays showed that Arabidopsis VQ proteins interacted specifically with the C-terminal WRKY domains of group I and the sole WRKY domains of group IIc WRKY proteins. Using site-directed mutagenesis, we identified structural features of these two closely related groups of WRKY domains that are critical for interaction with VQ proteins. Quantitative reverse transcription polymerase chain reaction revealed that expression of a majority of Arabidopsis VQ genes was responsive to pathogen infection and salicylic acid treatment. Functional analysis using both knockout mutants and overexpression lines revealed strong phenotypes in growth, development, and susceptibility to pathogen infection. Altered phenotypes were substantially enhanced through cooverexpression of genes encoding interacting VQ and WRKY proteins. These findings indicate that VQ proteins play an important role in plant growth, development, and response to environmental conditions, most likely by acting as cofactors of group I and IIc WRKY transcription factors. |
| Volume | 159(2) |
| Pages | 810-25 |
| Published | 2012-6-1 |
| DOI | 10.1104/pp.112.196816 |
| PII | pp.112.196816 |
| PMID | 22535423 |
| PMC | PMC3375943 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Botrytis / pathogenicity DNA, Plant / genetics DNA-Binding Proteins / metabolism Disease Resistance Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Mutagenesis, Site-Directed Phenotype Phylogeny Plant Diseases / immunology Plant Immunity Plant Leaves / genetics Plant Leaves / metabolism Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Promoter Regions, Genetic Protein Interaction Mapping* Pseudomonas syringae / pathogenicity Salicylic Acid / pharmacology Sigma Factor / genetics Sigma Factor / metabolism Structure-Activity Relationship Substrate Specificity Transcription Factors / genetics Transcription Factors / metabolism* Two-Hybrid System Techniques |
| IF | 6.902 |
| Times Cited | 107 |
| WOS Category | PLANT SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst10287 pst02467 pst13186 pst20793 |