論文 - 詳細
| RRC ID | 28119 |
|---|---|
| 著者 | Shinya T, Motoyama N, Ikeda A, Wada M, Kamiya K, Hayafune M, Kaku H, Shibuya N. |
| タイトル | Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Chitin is a representative microbe-associated molecular pattern (MAMP) molecule for various fungi and induces immune responses in many plant species. It has been clarified that the chitin signaling in rice requires a receptor kinase OsCERK1 and a receptor-like protein (Os)CEBiP, which specifically binds chitin oligosaccharides. On the other hand, Arabidopsis requires a receptor kinase (At)CERK1 for chitin signaling but it is not clear whether the plant also requires a CEBiP-like molecule for chitin perception/signaling. To clarify the similarity/difference of the chitin receptor in these two model plants, we first characterized CEBiP homologs in Arabidopsis. Only one of three CEBiP homologs, AtCEBiP (LYM2), showed a high-affinity binding for chitin oligosaccharides similar to rice CEBiP. AtCEBiP also represented the major chitin-binding protein in the Arabidopsis membrane. However, the single/triple knockout (KO) mutants of Arabidopsis CEBiP homologs and the overexpressor of AtCEBiP showed chitin-induced defense responses similar to wild-type Arabidopsis, indicating that AtCEBiP is biochemically functional as a chitin-binding protein but does not contribute to signaling. Studies of the chitin binding properties of the ectodomains of At/OsCERK1 and the chimeric receptors consisting of ecto/cytosolic domains of these molecules indicated that AtCERK1 is sufficient for chitin perception by itself. |
| 巻・号 | 53(10) |
| ページ | 1696-706 |
| 公開日 | 2012-10-1 |
| DOI | 10.1093/pcp/pcs113 |
| PII | pcs113 |
| PMID | 22891159 |
| MeSH | Arabidopsis / immunology Arabidopsis / metabolism* Arabidopsis / microbiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Chitin / metabolism* Cytosol / metabolism Gene Expression Regulation, Plant Gene Knockout Techniques Oryza / immunology Oryza / metabolism* Oryza / microbiology Plant Proteins / metabolism* Plants, Genetically Modified Protein Serine-Threonine Kinases / metabolism* Protein Structure, Tertiary Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Signal Transduction Tobacco / genetics Tobacco / metabolism |
| IF | 4.062 |
| 引用数 | 101 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | sja16500 |