RRC ID 28122
Author Carroll A, Mansoori N, Li S, Lei L, Vernhettes S, Visser RG, Somerville C, Gu Y, Trindade LM.
Title Complexes with mixed primary and secondary cellulose synthases are functional in Arabidopsis plants.
Journal Plant Physiol.
Abstract In higher plants, cellulose is synthesized by so-called rosette protein complexes with cellulose synthases (CESAs) as catalytic subunits of the complex. The CESAs are divided into two distinct families, three of which are thought to be specialized for the primary cell wall and three for the secondary cell wall. In this article, the potential of primary and secondary CESAs forming a functional rosette complex has been investigated. The membrane-based yeast two-hybrid and biomolecular fluorescence systems were used to assess the interactions between three primary (CESA1, CESA3, CESA6), and three secondary (CESA4, CESA7, CESA8) Arabidopsis (Arabidopsis thaliana) CESAs. The results showed that all primary CESAs can physically interact both in vitro and in planta with all secondary CESAs. Although CESAs are broadly capable of interacting in pairwise combinations, they are not all able to form functional complexes in planta. Analysis of transgenic lines showed that CESA7 can partially rescue defects in the primary cell wall biosynthesis in a weak cesa3 mutant. Green fluorescent protein-CESA protein fusions revealed that when CESA3 was replaced by CESA7 in the primary rosette, the velocity of the mixed complexes was slightly faster than the native primary complexes. CESA1 in turn can partly rescue defects in secondary cell wall biosynthesis in a cesa8ko mutant, resulting in an increase of cellulose content relative to cesa8ko. These results demonstrate that sufficient parallels exist between the primary and secondary complexes for cross-functionality and open the possibility that mixed complexes of primary and secondary CESAs may occur at particular times.
Volume 160(2)
Pages 726-37
Published 2012-10
DOI 10.1104/pp.112.199208
PII pp.112.199208
PMID 22926318
PMC PMC3461551
MeSH Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / physiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Wall / enzymology Cell Wall / metabolism Cellulose / metabolism Glucosyltransferases / genetics Glucosyltransferases / metabolism* Green Fluorescent Proteins / metabolism Multiprotein Complexes / metabolism Plant Leaves / enzymology Plant Leaves / physiology Plant Vascular Bundle / enzymology Plant Vascular Bundle / metabolism Plant Vascular Bundle / physiology Plants, Genetically Modified / enzymology Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Promoter Regions, Genetic Protein Interaction Mapping / methods Recombinant Fusion Proteins / metabolism Transcriptome Two-Hybrid System Techniques
IF 5.949
Times Cited 32
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda01408 pda09751 pda08626 pda13209 pda13285