RRC ID 28127
著者 Hozumi A, Mita K, Miskey C, Mates L, Izsvak Z, Ivics Z, Satake H, Sasakura Y.
タイトル Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element.
ジャーナル Dev Dyn
Abstract BACKGROUND:Transposon-mediated transgenesis is an excellent method for creating stable transgenic lines and insertional mutants. In the chordate Ciona intestinalis, Minos is the only transposon that has been used as the tool for germline transformation. Adding another transposon system in this organism enables us to conduct genetic techniques which can only be realized with the use of two transposons.
RESULTS:In the present study, we found that another Tc1/mariner superfamily transposon, sleeping beauty (SB), retains sufficient activity for germline transformation of C. intestinalis. SB shows efficiencies of germline transformation, insertion into gene coding regions, and enhancer detection comparable to those of Minos. We have developed a system for the remobilization of SB copies in the C. intestinalis genome by using transgenic lines expressing SB transposase in the germ cells. With this system, we examined the manner of SB mobilization in the C. intestinalis genome. SB shows intrachromosomal transposition more frequently than Minos.
CONCLUSIONS:SB-based germline transformation and the establishment of a new method that uses its frequent intrachromosomal transposition will result in breakthroughs in genetic approaches that use C. intestinalis together with Minos.
巻・号 242(1)
ページ 30-43
公開日 2013-1-1
DOI 10.1002/dvdy.23891
PMID 23073965
MeSH Animals Animals, Genetically Modified Ciona intestinalis / genetics* DNA Primers / genetics DNA Transposable Elements / genetics* Electroporation Gene Transfer Techniques* Germ Cells / metabolism* Green Fluorescent Proteins / metabolism Microinjections Reverse Transcriptase Polymerase Chain Reaction Transposases / metabolism
IF 3.275
引用数 8
WOS 分野 ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Tg[MiFr3dTPOCCiNutSB100x]1 Tg[MiFr3dTPOCCiNutSB100x]2 Tg[MiFr3dTPOCCiPrmSB100x]1 Tg[MiFr3dTPOCCiPrmSB100x]2 Tg[T2dTPOG]1 Tg[T2dTPOG]3 Wild C. int