RRC ID 28129
Author Hyodo H, Terao A, Furukawa J, Sakamoto N, Yurimoto H, Satoh S, Iwai H.
Title Tissue specific localization of pectin-Ca²⁺ cross-linkages and pectin methyl-esterification during fruit ripening in tomato (Solanum lycopersicum).
Journal PLoS ONE
Abstract Fruit ripening is one of the developmental processes accompanying seed development. The tomato is a well-known model for studying fruit ripening and development, and the disassembly of primary cell walls and the middle lamella, such as through pectin de-methylesterified by pectin methylesterase (PE) and depolymerization by polygalacturonase (PG), is generally accepted to be one of the major changes that occur during ripening. Although many reports of the changes in pectin during tomato fruit ripening are focused on the relation to softening of the pericarp or the Blossom-end rot by calcium (Ca²⁺) deficiency disorder, the changes in pectin structure and localization in each tissues during tomato fruit ripening is not well known. In this study, to elucidate the tissue-specific role of pectin during fruit development and ripening, we examined gene expression, the enzymatic activities involved in pectin synthesis and depolymerisation in fruit using biochemical and immunohistochemical analyses, and uronic acids and calcium (Ca)-bound pectin were determined by secondary ion-microprobe mass spectrometry. These results show that changes in pectin properties during fruit development and ripening have tissue-specific patterns. In particular, differential control of pectin methyl-esterification occurs in each tissue. Variations in the cell walls of the pericarp are quite different from that of locular tissues. The Ca-binding pectin and hairy pectin in skin cell layers are important for intercellular and tissue-tissue adhesion. Maintenance of the globular form and softening of tomato fruit may be regulated by the arrangement of pectin structures in each tissue.
Volume 8(11)
Pages e78949
Published 2013
DOI 10.1371/journal.pone.0078949
PII PONE-D-13-23131
PMID 24236073
PMC PMC3827314
MeSH Calcium / metabolism* Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism Cell Wall / metabolism Esterification Fruit / cytology Fruit / growth & development* Fruit / metabolism Gene Expression Lycopersicon esculentum / cytology Lycopersicon esculentum / growth & development* Lycopersicon esculentum / metabolism Organ Specificity Pectins / metabolism* Plant Epidermis / cytology Plant Epidermis / metabolism Plant Proteins / genetics Plant Proteins / metabolism Polygalacturonase / genetics Polygalacturonase / metabolism
IF 2.766
Times Cited 14
Tomato TOMJPF00001