RRC ID 28159
著者 Lepistö A, Pakula E, Toivola J, Krieger-Liszkay A, Vignols F, Rintamäki E.
タイトル Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods.
ジャーナル J Exp Bot
Abstract Plastid-localized NADPH-dependent thioredoxin reductase C (NTRC) is a unique NTR enzyme containing both reductase and thioredoxin domains in a single polypeptide. Arabidopsis thaliana NTRC knockout lines (ntrc) show retarded growth, especially under short-day (SD) photoperiods. This study identified chloroplast processes that accounted for growth reduction in SD-acclimated ntrc. The strongest reduction in ntrc growth occurred under photoperiods with nights longer than 14 h, whereas knockout of the NTRC gene did not alter the circadian-clock-controlled growth of Arabidopsis. Lack of NTRC modulated chloroplast reactive oxygen species (ROS) metabolism, but oxidative stress was not the primary cause of retarded growth of SD-acclimated ntrc. Scarcity of starch accumulation made ntrc leaves particularly vulnerable to photoperiods with long nights. Direct interaction of NTRC and ADP-glucose pyrophosphorylase, a key enzyme in starch synthesis, was confirmed by yeast two-hybrid analysis. The ntrc line was not able to maximize starch synthesis during the light period, which was particularly detrimental under SD conditions. Acclimation of Arabidopsis to SD conditions also involved an inductive rise of ROS production in illuminated chloroplasts that was not counterbalanced by the activation of plastidial anti-oxidative systems. It is proposed that knockout of NTRC challenges redox regulation of starch synthesis, resulting in stunted growth of the mutant lines acclimated to the SD photoperiod.
巻・号 64(12)
ページ 3843-54
公開日 2013-9-1
DOI 10.1093/jxb/ert216
PII ert216
PMID 23881397
PMC PMC3745738
MeSH Acclimatization Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / physiology* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Chloroplasts / genetics Chloroplasts / metabolism Glucose-1-Phosphate Adenylyltransferase / metabolism Oxidative Stress Photoperiod* Plant Leaves / genetics Plant Leaves / metabolism Reactive Oxygen Species / metabolism Starch / biosynthesis* Thioredoxin-Disulfide Reductase / genetics* Thioredoxin-Disulfide Reductase / metabolism
IF 5.908
引用数 43
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda04167 pda05951