Reference - Detail
| RRC ID | 28160 |
|---|---|
| Author | Begum P, Fugetsu B. |
| Title | Induction of cell death by graphene in Arabidopsis thaliana (Columbia ecotype) T87 cell suspensions. |
| Journal | J Hazard Mater |
| Abstract |
The toxicity of graphene on suspensions of Arabidopsis thaliana (Columbia ecotype) T87 cells was investigated by examining the morphology, mitochondrial dysfunction, reactive oxygen species generation (ROS), and translocation of graphene as the toxicological endpoints. The cells were grown in Jouanneau and Péaud-Lenoel (JPL) media and exposed to graphene at concentrations 0-80 mg/L. Morphological changes were observed by scanning electron microscope and the adverse effects such as fragmented nuclei, membrane damage, mitochondrial dysfunction was observed with fluorescence microscopy by staining with Hoechst 33342/propidium iodide and succinate dehydrogenase (mitochondrial bioenergetic enzyme). Analysis of intracellular ROS by 2',7'-dichlorofluorescein diacetate demonstrated that graphene induced a 3.3-fold increase in ROS, suggesting that ROS are key mediators in the cell death signaling pathway. Transmission electron microscopy verified the translocation of graphene into cells and an endocytosis-like structure was observed which suggested graphene entering into the cells by endocytosis. In conclusion, our results show that graphene induced cell death in T87 cells through mitochondrial damage mediated by ROS. |
| Volume | 260 |
| Pages | 1032-41 |
| Published | 2013-9-15 |
| DOI | 10.1016/j.jhazmat.2013.06.063 |
| PII | S0304-3894(13)00463-9 |
| PMID | 23892171 |
| MeSH | Apoptosis / drug effects* Arabidopsis / drug effects* Arabidopsis / ultrastructure Benzimidazoles / chemistry Cell Membrane / drug effects Cell Nucleus / drug effects Cells, Cultured Culture Media / chemistry Dose-Response Relationship, Drug Endocytosis Graphite / chemistry Graphite / toxicity* Membrane Potential, Mitochondrial / drug effects Microscopy, Atomic Force Microscopy, Fluorescence Oxidative Stress Propidium / chemistry Reactive Oxygen Species Succinate Dehydrogenase / chemistry Water / chemistry |
| IF | 9.038 |
| Times Cited | 37 |
| WOS Category | ENGINEERING, CIVIL ENVIRONMENTAL SCIENCES ENGINEERING, ENVIRONMENTAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00008 |