RRC ID 2824
著者 Ikeguchi R, Kakinoki R, Matsumoto T, Yamakawa T, Nakayama K, Morimoto Y, Nakamura T.
タイトル Successful storage of peripheral nerves using University of Wisconsin solution with polyphenol.
ジャーナル J Neurosci Methods
Abstract We have previously reported that green tea polyphenol can preserve peripheral nerve segments for up to 1 month. In this study, we investigated the effect on peripheral nerve preservation of adding polyphenol to the conventional University of Wisconsin solution (UW solution), which has been widely used for organ storage. Twenty millimeter-long sciatic nerve segments, harvested from Lewis rats, were immersed in UW solution containing polyphenol (1 mg/mL) for 1 week and then in UW solution alone at 4 degrees C for 3 additional weeks before transplantation into recipient Lewis rats. Neural cell viability of the preserved nerve segments was confirmed by vital staining (calcein-AM/ethidium homodimer), electron microscopy, and genomic studies. Morphologically, nerve regeneration was similar to that of fresh isografts and superior to that of grafts stored with UW solution alone. Moreover, the electrophysiological results were equal to those of fresh isografts. Polyphenol has the potential to be used for peripheral nerve storage and could be useful for routine peripheral nerve banking.
巻・号 159(1)
ページ 57-65
公開日 2007-1-15
DOI 10.1016/j.jneumeth.2006.06.025
PII S0165-0270(06)00309-8
PMID 16942801
MeSH Actins / genetics Animals Axons / physiology DNA / biosynthesis DNA / genetics Electric Stimulation Electrophysiology Female Flavonoids / pharmacology* Image Processing, Computer-Assisted Male Microscopy, Electron Nerve Fibers, Myelinated / physiology Nerve Regeneration Peripheral Nerves / physiology* Peripheral Nerves / transplantation Phenols / pharmacology* Polyphenols Rats Rats, Inbred Lew Reverse Transcriptase Polymerase Chain Reaction Sciatic Nerve / physiology Sciatic Nerve / transplantation Solutions Tea / chemistry Tissue Preservation / methods*
IF 2.214
引用数 2
WOS 分野 NEUROSCIENCES BIOCHEMICAL RESEARCH METHODS
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