RRC ID |
2824
|
著者 |
Ikeguchi R, Kakinoki R, Matsumoto T, Yamakawa T, Nakayama K, Morimoto Y, Nakamura T.
|
タイトル |
Successful storage of peripheral nerves using University of Wisconsin solution with polyphenol.
|
ジャーナル |
J Neurosci Methods
|
Abstract |
We have previously reported that green tea polyphenol can preserve peripheral nerve segments for up to 1 month. In this study, we investigated the effect on peripheral nerve preservation of adding polyphenol to the conventional University of Wisconsin solution (UW solution), which has been widely used for organ storage. Twenty millimeter-long sciatic nerve segments, harvested from Lewis rats, were immersed in UW solution containing polyphenol (1 mg/mL) for 1 week and then in UW solution alone at 4 degrees C for 3 additional weeks before transplantation into recipient Lewis rats. Neural cell viability of the preserved nerve segments was confirmed by vital staining (calcein-AM/ethidium homodimer), electron microscopy, and genomic studies. Morphologically, nerve regeneration was similar to that of fresh isografts and superior to that of grafts stored with UW solution alone. Moreover, the electrophysiological results were equal to those of fresh isografts. Polyphenol has the potential to be used for peripheral nerve storage and could be useful for routine peripheral nerve banking.
|
巻・号 |
159(1)
|
ページ |
57-65
|
公開日 |
2007-1-15
|
DOI |
10.1016/j.jneumeth.2006.06.025
|
PII |
S0165-0270(06)00309-8
|
PMID |
16942801
|
MeSH |
Actins / genetics
Animals
Axons / physiology
DNA / biosynthesis
DNA / genetics
Electric Stimulation
Electrophysiology
Female
Flavonoids / pharmacology*
Image Processing, Computer-Assisted
Male
Microscopy, Electron
Nerve Fibers, Myelinated / physiology
Nerve Regeneration
Peripheral Nerves / physiology*
Peripheral Nerves / transplantation
Phenols / pharmacology*
Polyphenols
Rats
Rats, Inbred Lew
Reverse Transcriptase Polymerase Chain Reaction
Sciatic Nerve / physiology
Sciatic Nerve / transplantation
Solutions
Tea / chemistry
Tissue Preservation / methods*
|
IF |
2.214
|
引用数 |
2
|
WOS 分野
|
NEUROSCIENCES
BIOCHEMICAL RESEARCH METHODS
|
リソース情報 |
ラット |
|