| RRC ID |
2826
|
| 著者 |
Enomoto A, Kikuchi T, Seo N, Matsuno K, Kobayashi E.
|
| タイトル |
Impact of cold preservation on leukocyte adhesion to the transplanted rat lung.
|
| ジャーナル |
Microsurgery
|
| Abstract |
Little is known about leukocyte migration in the early phase after ischemia-reperfusion injury. Recently, there has been much focus on in vivo visualization of cell kinetics. Previously, we established a method for the in vivo visualization of leukocyte circulation using a Green Fluorescent Protein transgenic rat. Herein, we developed a simplified rat heterotopic lung transplantation model that can be mastered by beginners within a few weeks. Using this system, we studied the impact of preservation on adhesion of circulating leukocytes to the transplanted lung. The graft of a fresh group was compared with that of a preserved group. Morphological and immunohistochemical analyses were also performed. We successfully visualized few adherent leukocytes in the fresh graft, whereas adherent cells were attached in the preserved graft within a few minutes. In conclusion, the prolonged cold preservation time promotes leukocyte adhesion, resulting in increased microvascular injury after lung transplantation.
|
| 巻・号 |
27(4)
|
| ページ |
228-33
|
| 公開日 |
2007-1-1
|
| DOI |
10.1002/micr.20341
|
| PMID |
17477404
|
| MeSH |
Animals
Animals, Genetically Modified
Cell Adhesion / physiology
Cryopreservation*
Green Fluorescent Proteins / metabolism
Leukocytes / physiology*
Lung Transplantation*
Male
Microcirculation
Models, Animal
Random Allocation
Rats
Rats, Inbred Lew
Reperfusion Injury / physiopathology*
|
| IF |
1.996
|
| 引用数 |
2
|
|
WOS 分野
|
SURGERY
|
| リソース情報 |
| ラット |
LEW-Tg(CAG-EGFP)1Ys(strainID=647) |
