論文 - 詳細
| RRC ID | 2828 |
|---|---|
| 著者 | Anas C, Ozaki T, Maruyama S, Yamamoto T, Zu Gotoh M, Ono Y, Matsuo S. |
| タイトル | Effects of olprinone, a phosphodiesterase III inhibitor, on ischemic acute renal failure. |
| ジャーナル | Int J Urol |
| Abstract |
OBJECTIVE:Renal ischemic reperfusion injury (IRI) is unavoidable and is still one of the major problems in renal transplantation. The aim of this study was to investigate the effects of olprinone, a phosphodiesterase III inhibitor, on renal IRI. METHODS:After a right nephrectomy, renal IRI was induced in rats. Olprinone was given in two different ways: sustained systemic administration and transient local administration to the kidney. Control rats were treated with saline. Using a magnifying endoscope, the renal blood flow speed was measured at 23 h after reperfusion. Then, blood samples were collected, and kidney specimens were taken for histological study. In order to study the mechanism, we performed in vitro experiments, using human proximal renal tubular cells (HK-2) incubated with tumor necrosis factor (TNF)-alpha along with olprinone or saline, and interleukin (IL)-8 was measured in the culture supernatant. RESULTS:In the saline group, the blood flow speed (BFS) was greatly reduced compared to that in normal kidneys. In both olprinone-treated groups, BFS of the renal microcirculation significantly increased, tubular damage and macrophage infiltration attenuated, and renal function greatly improved. Olprinone inhibited the increase in the IL-8 levels resulting from the incubation of HK-2 with TNF-alpha. CONCLUSIONS:Our study successfully demonstrates that olprinone has renoprotective properties when applied locally as well as systemically. The results suggest that olprinone might be clinically useful in renal transplantation for the donor kidney, the recipient, and even in treating acute renal failure. |
| 巻・号 | 14(3) |
| ページ | 219-25 |
| 公開日 | 2007-3-1 |
| DOI | 10.1111/j.1442-2042.2007.01689.x |
| PII | IJU1689 |
| PMID | 17430259 |
| MeSH | 3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors* 3',5'-Cyclic-AMP Phosphodiesterases / blood Acute Kidney Injury / drug therapy* Acute Kidney Injury / etiology Acute Kidney Injury / metabolism Animals Blood Flow Velocity Blood Urea Nitrogen Cells, Cultured Cyclic Nucleotide Phosphodiesterases, Type 3 Disease Models, Animal Humans Imidazoles / therapeutic use* Immunohistochemistry Interleukin-8 / metabolism Kidney Tubules, Proximal / drug effects Kidney Tubules, Proximal / metabolism Kidney Tubules, Proximal / pathology Male Microscopy, Video Phosphodiesterase Inhibitors / therapeutic use* Pyridones / therapeutic use* Rats Rats, Sprague-Dawley Renal Circulation / drug effects Reperfusion Injury / complications* Reperfusion Injury / drug therapy Reperfusion Injury / physiopathology Treatment Outcome |
| IF | 2.445 |
| 引用数 | 11 |
| WOS 分野 | UROLOGY & NEPHROLOGY |
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