RRC ID 2837
著者 Iida A, Shimada A, Shima A, Takamatsu N, Hori H, Takeuchi K, Koga A.
タイトル Targeted reduction of the DNA methylation level with 5-azacytidine promotes excision of the medaka fish Tol2 transposable element.
ジャーナル Genet Res
Abstract The Tol2 element of the medaka fish Oryzias latipes is a member of the hAT (hobo/Activator/Tam3) transposable element family. There is evidence for rapid expansion in the genome and throughout the species in the past but a high spontaneous transposition rate is not observed with current fish materials, suggesting that the Tol2 element and its host species have already acquired an interactive mechanism to control the transposition frequency. DNA methylation is a possible contributing factor, given its involvement with many other transposable elements. We therefore soaked embryos in 5-azacytidine, a reagent that causes reduction in the DNA methylation level, and examined amounts of PCR products reflecting the somatic excision frequency, obtaining direct evidence that exposure promotes Tol2 excision. Our results thus suggest that methylation of the genome DNA is a factor included in the putative mechanisms of control of transposition of the Tol2 element.
巻・号 87(3)
ページ 187-93
公開日 2006-6-1
DOI 10.1017/S0016672306008184
PII S0016672306008184
PMID 16818001
MeSH Animals Azacitidine / pharmacology* DNA Methylation / drug effects* DNA Transposable Elements* / drug effects Embryo, Nonmammalian / drug effects Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental / drug effects Models, Genetic Oryzias / embryology Oryzias / genetics* Oryzias / metabolism Transposases / metabolism
IF 1.167
引用数 14
WOS 分野 GENETICS & HEREDITY
リソース情報
メダカ NA