| RRC ID |
28437
|
| Author |
Nikaido T, Shimizu A, Ishida N, Sabe H, Teshigawara K, Maeda M, Uchiyama T, Yodoi J, Honjo T.
|
| Title |
Molecular cloning of cDNA encoding human interleukin-2 receptor.
|
| Journal |
Nature
|
| Abstract |
The human interleukin-2 (IL-2) receptor was purified by affinity chromatography using the anti-Tac monoclonal antibody, and its N-terminal amino acid sequence was determined. Complementary DNA clones were isolated and sequenced to reveal the primary structure of the IL-2 receptor precursor, which has 272 amino acid residues. The receptor is separated into two domains by a putative 19-residue transmembrane region. Two mRNAs (1.4 and 3.5 kilobases) hybridizing to the cDNA clone were found in human T cells bearing the IL-2 receptor. The cDNA directed synthesis of the IL-2 receptor in COS cells.
|
| Volume |
311(5987)
|
| Pages |
631-5
|
| Published |
1984-10-18
|
| DOI |
10.1038/311631a0
|
| PMID |
6090949
|
| MeSH |
Amino Acid Sequence
Base Sequence
Cloning, Molecular*
DNA / isolation & purification
DNA / metabolism*
DNA Restriction Enzymes
Genes*
Genetic Vectors
Humans
Nucleic Acid Hybridization
Protein Biosynthesis
RNA, Messenger / genetics
Receptors, Antigen, T-Cell / genetics*
Receptors, Immunologic / genetics*
Receptors, Immunologic / isolation & purification
Receptors, Interleukin-2
Transcription, Genetic
|
| IF |
42.779
|
| Times Cited |
601
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pKCR. Tac-2A (RDB01171) |
