RRC ID 28440
Author Kato H, Sakai T, Tamura K, Minoguchi S, Shirayoshi Y, Hamada Y, Tsujimoto Y, Honjo T.
Title Functional conservation of mouse Notch receptor family members.
Journal FEBS Lett
Abstract All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr virus nuclear antigen 2. Furthermore the intracellular region of int3 was shown to transactivate the Epstein Barr virus TP1 promoter. These results indicate that all mouse Notch family members have biochemical functions similar to mNotch1, which transduces proliferative signal by direct interaction with the DNA binding protein RBP-Jkappa.
Volume 395(2-3)
Pages 221-4
Published 1996-10-21
DOI 10.1016/0014-5793(96)01046-0
PII 0014-5793(96)01046-0
PMID 8898100
MeSH Amino Acid Sequence Animals Base Sequence Conserved Sequence DNA Primers Epstein-Barr Virus Nuclear Antigens / chemistry Epstein-Barr Virus Nuclear Antigens / metabolism Glutathione Transferase Herpesvirus 4, Human / genetics Membrane Proteins / biosynthesis* Membrane Proteins / chemistry* Mice Molecular Sequence Data Polymerase Chain Reaction Promoter Regions, Genetic Receptors, Cell Surface / biosynthesis Receptors, Cell Surface / chemistry Receptors, Notch Recombinant Fusion Proteins / biosynthesis Sequence Homology, Amino Acid
IF 3.057
Times Cited 86
DNA material pEF-BOSneo-mNotch1 RAMIC (RDB06771) pEF-BOSneoSE-mNotch2 RAMIC (RDB06772) pEF-BOSneoSE-mNotch3 RAMIC (RDB06773) pEF-BOSneo-mNotch4 RAMIC (RDB06774).