RRC ID 28443
Author Nagafuchi A, Shirayoshi Y, Okazaki K, Yasuda K, Takeichi M.
Title Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA.
Journal Nature
Abstract E-cadherin is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells; it is also called uvomorulin, L-CAM (ref. 3), cell-CAM 120/80 (ref.4) or Arc-1 (ref. 5). Because blocking the action of E-cadherin by monoclonal antibodies causes dispersion of compact cell colonies, this molecule is thought to be an important factor for maintenance of multicellular systems. To demonstrate directly that E-cadherin is involved in cell-cell adhesion, we cloned full-length cDNA encoding E-cadherin from F9 cells and introduced it into L fibroblasts deficient in E-cadherin. These L cells acquire strong Ca2+-dependent aggregating activity by expressing the E-cadherin derived from the introduced cDNA and were morphologically transformed so as to form colonies in which cells were tightly connected to each other.
Volume 329(6137)
Pages 341-3
Published 1987-9-24
DOI 10.1038/329341a0
PMID 3498123
MeSH Amino Acid Sequence Animals Base Sequence Cadherins Calcium / pharmacology Cell Adhesion Cell Line DNA / genetics* DNA, Recombinant Glycoproteins / genetics Glycoproteins / physiology* L Cells (Cell Line) / cytology Mice RNA, Messenger / genetics Teratoma Transfection Transformation, Genetic*
IF 41.577
Times Cited 647
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material Mouse E-cadherin full length cDNA (RDB01184).