| RRC ID |
28445
|
| 著者 |
Hatta M, Miyatani S, Copeland NG, Gilbert DJ, Jenkins NA, Takeichi M.
|
| タイトル |
Genomic organization and chromosomal mapping of the mouse P-cadherin gene.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
Cadherins are a family of Ca(2+)-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45 kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23 kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the correspoding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes.
|
| 巻・号 |
19(16)
|
| ページ |
4437-41
|
| 公開日 |
1991-8-25
|
| DOI |
10.1093/nar/19.16.4437
|
| PMID |
1886768
|
| PMC |
PMC328631
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cadherins / genetics*
Cell Adhesion Molecules / genetics
Chickens
Chromosome Mapping
Cloning, Molecular
Crosses, Genetic
Exons / genetics
Introns / genetics
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Multigene Family / genetics*
|
| IF |
11.502
|
| 引用数 |
66
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
Mouse P-cadherin full length cDNA (RDB01185). |
