論文 - 詳細
| RRC ID | 28458 |
|---|---|
| 著者 | Takaine M, Numata O, Nakano K. |
| タイトル | Fission yeast IQGAP maintains F-actin-independent localization of myosin-II in the contractile ring. |
| ジャーナル | Genes Cells |
| Abstract |
During cytokinesis in many eukaryotic cells, myosin-II concentrates at the equatorial cortex with actin filaments (F-actin) and is supposed to generate forces to divide the cell into two, which is called the contractile ring (CR) hypothesis. Several lines of evidence indicate that the myosin-II is recruited independently of F-actin and interacts specifically with the equatorial F-actin. Molecular details of these mechanisms are still unknown. We used the fission yeast Schizosaccharomyces pombe to investigate the regulation of myosin-II localization. We demonstrate that the CR myosin-II was composed of F-actin-dependent and -independent fractions by simultaneously observing F-actin and myosin. The F-actin-independent fraction was visualized as cortical dots in the absence of F-actin. IQGAP Rng2, an indispensable element of CR, was implicated in maintenance of the F-actin-independent fraction of myosin-II, whereas anillin Mid1 was required for assembly but not for maintenance of the fraction. In the CR of the rng2 mutant, myosin-II was less concentrated, unstable, and nonhomogeneous, which often resulted in cytokinesis failure. These results suggest that Rng2 tethers myosin-II to the cortex along the CR independently of F-actin to provide a sufficient concentration. The robust localization of myosin-II would ensure successful cytokinesis. |
| 巻・号 | 19(2) |
| ページ | 161-76 |
| 公開日 | 2014-2-1 |
| DOI | 10.1111/gtc.12120 |
| PMID | 24330319 |
| MeSH | Actins / metabolism* Contractile Proteins / metabolism Myosin Type II / metabolism* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism |
| IF | 1.655 |
| 引用数 | 13 |
| WOS 分野 | GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 酵母 | FY14682 |