RRC ID 2855
著者 Koga A, Iida A, Hori H, Shimada A, Shima A.
タイトル Vertebrate DNA transposon as a natural mutator: the medaka fish Tol2 element contributes to genetic variation without recognizable traces.
ジャーナル Mol Biol Evol
Abstract DNA-based transposable elements, or DNA transposons, transpose in a cut-and-paste fashion, involving excision from the chromosome. If this process affects the function of a host gene and the excision rate is high, any gene associated with such an element would clearly be in a genetically "unstable" state, and there are many examples of unstable genes in various organisms. However, none have hitherto been reported in vertebrates. We here document the finding of an unstable mutant gene in the medaka fish, Oryzias latipes, a useful model animal for vertebrate genetics and evolutionary studies. In an inbred strain, excision of the Tol2 element inserted in a pigmentation gene occurs spontaneously, giving rise to different heritable phenotypes and new mutant genes that carry different excision footprint sequences. The phenotypic mutation rate is as high as 2% per gamete, representing a 1000-fold increase from spontaneous mutation rates so far determined with the same organism. With mutations caused by insertion, and then excision, of transposons, one can no longer recognize participation of transposons in their generation. Thus, the impact of DNA transposons on vertebrate genomes may be, and may have been, larger than commonly supposed.
巻・号 23(7)
ページ 1414-9
公開日 2006-7-1
DOI 10.1093/molbev/msl003
PII msl003
PMID 16672286
MeSH Alleles Animals Base Sequence Blotting, Southern Breeding DNA / analysis DNA / genetics DNA Transposable Elements / genetics* Embryo, Nonmammalian / metabolism Female Gene Frequency Genetic Variation / genetics* Male Molecular Sequence Data Mutagenesis, Insertional / genetics Mutation Oryzias / embryology Oryzias / genetics* Polymerase Chain Reaction Sequence Homology, Nucleic Acid
IF 11.062
引用数 30
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY EVOLUTIONARY BIOLOGY
リソース情報
メダカ i (i-1) (MT67) HdrR-II1 (IB178)